PLGA-磷酸钙复合载药纳米粒的制备

梁欢; 王倩; 王康; 刘新荣; 魏桂花; 晏为力; 周绍兵

doi:10.11777/j.issn1000-3304.2016.16032

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PLGA-磷酸钙复合载药纳米粒的制备

研究论文 | 更新时间：2021-03-19

- PLGA-磷酸钙复合载药纳米粒的制备
- Fabrication of Drug Loaded Nanoparticles of PLGA/Calcium Phosphate Composite
- 高分子学报 2016年第10期页码：1383-1389
- 作者机构：
  
  1.西南交通大学生命科学与工程学院成都 610031
  2.西南交通大学材料科学与工程学院成都 610031
- 作者简介：
  
  晏为力,E-mail:yanweili@gmail.com Wei-li Yan,E-mail:yanweili@gmail.com
  周绍兵,E-mail:shaobingzhou@swjtu.edu.cn Shao-bing Zhou,E-mail:shaobingzhou@swjtu.edu.cn
- 基金信息：
  
  四川省科技厅项目(项目号2015JY0202);国家自然科学基金(基金号21574105);四川省高校创新研究团队建设项目(项目号14TD0050);成都市科技惠民项目(项目号2015-HM01-00047-SF)
- DOI：10.11777/j.issn1000-3304.2016.16032
  中图分类号：
- 纸质出版日期：2016-10，
  
  收稿日期：2016-1-25，
  
  修回日期：2016-3-11，
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梁欢, 王倩, 王康, 刘新荣, 魏桂花, 晏为力, 周绍兵. PLGA-磷酸钙复合载药纳米粒的制备[J]. 高分子学报, 2016,(10):1383-1389.

Liang Huan, Wang Qian, Wang Kang, Liu Xin-rong, Wei Gui-hua, Yan Wei-li, Zhou Shao-bing. Fabrication of Drug Loaded Nanoparticles of PLGA/Calcium Phosphate Composite[J]. Acta Polymerica Sinica, 2016,(10):1383-1389.
梁欢, 王倩, 王康, 刘新荣, 魏桂花, 晏为力, 周绍兵. PLGA-磷酸钙复合载药纳米粒的制备[J]. 高分子学报, 2016,(10):1383-1389. DOI： 10.11777/j.issn1000-3304.2016.16032.

Liang Huan, Wang Qian, Wang Kang, Liu Xin-rong, Wei Gui-hua, Yan Wei-li, Zhou Shao-bing. Fabrication of Drug Loaded Nanoparticles of PLGA/Calcium Phosphate Composite[J]. Acta Polymerica Sinica, 2016,(10):1383-1389. DOI： 10.11777/j.issn1000-3304.2016.16032.

摘要

利用聚氧乙烯硬脂酸酯（Brij78）和帕米膦酸二钠制备新型表面活性剂Pa-Brij78，以此为表面活性剂，聚乳酸-羟基乙酸共聚物（poly（lactic-

-glycolic acid），PLGA）为油相，采用水包油包水的微乳液法制备载卵清蛋白（ovalbumin，OVA）的表面带有磷酸根的PLGA纳米粒，再用共沉淀法在其表面修饰一层磷酸钙，并装载寡核苷酸CpG，形成一种核-壳结构的复合载药纳米粒CpG/CaP/PLGA（OVA）.通过动态光散射粒度仪、扫描电子显微镜（SEM）、透射电子显微镜（TEM）、X射线衍射（XRD）对纳米粒进行表征，并测定OVA、CpG的载药量、包封率.结果表明以Pa-Brij78为表面活性剂制备的PLGA（OVA）纳米粒确实能被磷酸钙修饰，粒径增大40~60 nm，表面变粗糙，XRD测得该磷酸钙层的主要存在形式为Ca

（PO

）

.OVA平均载药量为5%，包封率大于80%；CpG平均载药量为0.47%，平均包封率为89.9%.

Abstract

Pamidronate was conjugated to Brij78 to prepare a novel surfactant Pa-Brij78. Using Pa-Brij78 as emulsifier and stabilizer

PLGA(OVA) nanoparticles with a negative charge were prepared by a water-oil-water double emulsion method. Calcium phosphate coated PLGA(OVA) nanoparticles were fabricated by a co-precipitation method. Toll like receptor ligand CpG was then coated on the surface of the nanoparticles to form CpG/CaP/PLGA(OVA)

a novel type of drug-loaded core-shell nanoparticles. The nanoparticles were characterized by Dynamic light scattering (DLS) particle size analyzer

scanning electron microscopy (SEM)

transmission electron microscopy (TEM) and X-ray diffraction. The loading capacity and encapsulation efficiency of OVA and CpG were determined. The results of SEM and TEM showed PLGA(OVA) nanoparticles had spherical shape with smooth and even surface

while CaP/PLGA(OVA) nanoparticles were spherical but their surface was rough and irregular due to uneven distribution of calcium phosphate. The coated shell of calcium phosphate was Ca

(PO

)

as confirmed by X-ray diffraction analysis. Average loading efficiency of OVA was 5% when encapsulation efficiency of OVA was above 80%. Average loading efficiency of CpG was 0.47% when average encapsulation efficiency of CpG was 89%.

关键词

可降解高分子磷酸钙核-壳结构复合载药纳米粒

Keywords

Biodegradable polymerCalcium phosphateCore-shell structureComposite drug loaded nanoparticles

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