水溶性共轭聚合物在生物传感中的应用

张恩东; 刘礼兵; 吕凤婷; 王树

doi:10.11777/j.issn1000-3304.2018.17269

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水溶性共轭聚合物在生物传感中的应用

专论 | 更新时间：2021-01-26

- 水溶性共轭聚合物在生物传感中的应用
- Water-soluble Conjugated Polymers for Biosensor Applications
- 高分子学报 2018年第2期页码：186-197
- 作者机构：
  
  1.中国科学院化学研究所北京 100190
  2.中国科学院大学北京 100049
- 作者简介：
  
  [ "王树, 男, 1972年出生.1990~1994年河北大学化学系, 学士; 1994~1999年北京大学化学与分子工程学院, 博士; 1999~2001年中国科学院化学研究所有机固体重点实验室博士后; 2001~2004年美国加利福尼亚大学圣巴巴拉分校博士后.2004年入选中国科学院百人计划, 任中国科学院化学研究所研究员, 博士生导师.主要研究方向:光功能有机共轭分子体系的设计、合成与生物应用.曾获中国化学会青年化学奖(2006年)、国家杰出青年基金(2007年)、中国科学院青年科学家奖(2011年)、第三届中国化学会-英国皇家化学会青年化学奖(2011年)、第十二届中国青年科技奖(2011年)、入选科技部创新领军人才(2013年)、北京市科学技术一等奖、入选国家百千万人才工程(2015年)以及国家\"万人计划\"领军人才(2016年), 获国务院政府特殊津贴(2016年)" ]
- 基金信息：
  
  国家自然科学基金(91527306)
- DOI：10.11777/j.issn1000-3304.2018.17269
  中图分类号：
- 纸质出版日期：2018-2-20，
  
  收稿日期：2017-9-18，
  
  修回日期：2017-11-2，
扫描看全文
张恩东, 刘礼兵, 吕凤婷, 王树. 水溶性共轭聚合物在生物传感中的应用[J]. 高分子学报, 2018,(2):186-197.

En-dong Zhang, Li-bing Liu, Feng-ting Lv, Shu Wang. Water-soluble Conjugated Polymers for Biosensor Applications[J]. Acta Polymerica Sinica, 2018,(2):186-197.
张恩东, 刘礼兵, 吕凤婷, 王树. 水溶性共轭聚合物在生物传感中的应用[J]. 高分子学报, 2018,(2):186-197. DOI： 10.11777/j.issn1000-3304.2018.17269.

En-dong Zhang, Li-bing Liu, Feng-ting Lv, Shu Wang. Water-soluble Conjugated Polymers for Biosensor Applications[J]. Acta Polymerica Sinica, 2018,(2):186-197. DOI： 10.11777/j.issn1000-3304.2018.17269.

摘要

水溶性共轭聚合物在化学、医学、生命科学以及材料科学等领域中备受研究者们的关注，利用其独特的光化学和光物理性质，研究者们开展了一系列创新性研究并取得了重大的研究进展，进而拓展了聚合物的应用范围.共轭聚合物是一种由多个重复发光单元通过彼此间共轭而形成的高分子化合物，通过对其结构的精准调控，可以获得具有不同性能的功能性分子.其中，通过改变其主链结构，可以获得具有不同吸收和发射波长的荧光探针；通过对侧链结构修饰以水溶性基团和/或选择性识别分子，可以实现与特定靶标间的静电作用或者特异性结合，进而实现选择性识别的目的.本文综述了近年来水溶性共轭聚合物在生物传感中的应用，主要介绍了水溶性共轭聚合物在DNA检测、蛋白质检测、细胞和细菌的检测与区分以及细胞成像等方面的研究进展.

Abstract

In recent years

conjugated polymers (CPs) with characteristic and excellent photochemical and photophysical properties have become particularly popular in scientific research and are widely used for biological sensing. Conjugated polymers are characterized by a delocalized electronic structure along their backbones and exhibit electronic coupling between each optoelectronic segments. Owing to unique delocalized

-conjugated structure

CPs are endowed with distinguished optical properties

such as light-harvesting and amplification capability and high energy transfer efficiency. Water-soluble conjugated polymers (WSCPs) introduced with charged groups or selectively-recognition elements on the side chains can specifically diagnose through electrostatic interaction or specific binding with targets. In addition

WSCPs with diverse absorption and emission characteristics have been designed and synthesized by tuning the constitution and conformation of the backbones. In contrast with fluorescence proteins

small organic molecules and quantum dots

CPs with greatly unique properties have greater advantages that directed to sensing and imaging in biochemical and biomedical fields. This review article is aimed to summarize recent research progresses on applications of WSCPs as biosensors for the detection of a series of biological targets. Firstly

according to electrostatics interaction of CPs and targeted DNA

different novel strategies are used for detection of DNA mutations (section 2). Meanwhile

CPs modified with recognition moieties can be utilized to detect specific proteins (section 3). In addition

based on different interaction modes between CPs and cells and pathogens

identification and discrimination of microbial pathogens and cells are realized (section 4). CPs can also be used for cell imaging due to their advantageous optical properties (section 5). The structures

characteristics and advantages of CPs

as well as various nove lidentifications and detections strategies are also discussed. Through a variety of design and modification

novel CPs can be obtained and used for identification and detection

which is complementary to traditional CPs in biosensor and widens the range of biological applications. It is worth mentioning that

with the development of inter-discipline

more attention should be paid to the combination of new methods and strategies for exploiting functions of CPs in biological sensing.

关键词

水溶性共轭聚合物生物传感信号放大识别检测生物成像

Keywords

Conjugated polymerBiosensorSignal amplificationIdentification and determinationCell imaging

references

J R Lakowicz , P A Koen , H Szmacinski , I Gryczynski , J Kuśba . J Fluoresc , 1994 . 4 ( 1 ): 117 - 136 . DOI:10.1007/BF01876666http://doi.org/10.1007/BF01876666.

F de Lange , A Cambi , R Huijbens , B de Bakker , W Rensen , M Garcia-Parajo , N van Hulst , C G Figdor . J Cell Sci , 2001 . 114 4153 - 4160 . http://www.ncbi.nlm.nih.gov/pubmed/11739648.

M Fernández-Suárez , A Y Ting . Nat Rev Mol Cell Biol , 2008 . 9 929 - 943 . DOI:10.1038/nrm2531http://doi.org/10.1038/nrm2531.

S Weiss . Science , 1999 . 283 ( 5408 ): 1676 - 1683 . DOI:10.1126/science.283.5408.1676http://doi.org/10.1126/science.283.5408.1676.

X Ge , A Leah Tolosa , G Rao . Anal Chem , 2004 . 76 ( 5 ): 1403 - 1410 . DOI:10.1021/ac035063phttp://doi.org/10.1021/ac035063p.

T Xia , N Li , X H Fang . Annu Rev Phys Chem , 2013 . 64 459 - 480 . DOI:10.1146/annurev-physchem-040412-110127http://doi.org/10.1146/annurev-physchem-040412-110127.

K Nienhaus , G U Nienhaus . Chem Soc Rev , 2014 . 43 1088 - 1106 . DOI:10.1039/C3CS60171Dhttp://doi.org/10.1039/C3CS60171D.

K Rombouts , K Braeckmans , K Remaut . Bioconjugate Chem , 2016 . 27 ( 2 ): 280 - 297 . http://www.ncbi.nlm.nih.gov/pubmed/26670733.

J Zhou , Y Yang , C Y Zhang . Chem Rev , 2015 . 115 11669 - 11717 . DOI:10.1021/acs.chemrev.5b00049http://doi.org/10.1021/acs.chemrev.5b00049.

J Mei , N L C Leung , R T K Kwok , J W Y Lam , B Z Tang . Chem Rev , 2015 . 115 11718 - 11940 . DOI:10.1021/acs.chemrev.5b00263http://doi.org/10.1021/acs.chemrev.5b00263.

T Ito , H Shirakawa , S Ikeda . J Polym Sci, Part A:Polym Chem , 1996 . 34 ( 13 ): 2533 - 2542 . DOI:10.1002/(ISSN)1099-0518http://doi.org/10.1002/(ISSN)1099-0518.

C K Chiang , C R J Fincher , Y W Park , A J Heeger , H Shirakawa , E J Louis , S C Gau , A G MacDiarmid . Phys Rev Lett , 1977 . 39 ( 17 ): 1098 - 1101 . DOI:10.1103/PhysRevLett.39.1098http://doi.org/10.1103/PhysRevLett.39.1098.

X Feng , L Liu , S Wang , D Zhu . Chem Soc Rev , 2010 . 39 ( 7 ): 2411 - 2419 . DOI:10.1039/b909065ghttp://doi.org/10.1039/b909065g.

C L Zhu , L B Liu , Q Yang , F T Lv , S Wang . Chem Rev , 2012 . 112 4687 - 4735 . DOI:10.1021/cr200263whttp://doi.org/10.1021/cr200263w.

H X Yuan , B Wang , F T Lv , L B Liu , S Wang . Adv Mater , 2016 . 26 ( 40 ): 6978 - 6982 . http://www.ncbi.nlm.nih.gov/pubmed/24711269.

Jiangyan Zhang , Huanxiang Yuan , Chunlei Zhu , Libing Liu , Fengting Lv , Shu Wang . Scientia Sinca Chimica , 2016 . 46 ( 2 ): 153 - 162 . http://dx.doi.org/10.1360/N032015-00122.

张江艳 , 袁焕祥 , 朱春雷 , 刘礼兵 , 吕凤婷 , 王树 . 中国科学化学 , 2016 . 46 ( 2 ): 153 - 162 . http://dx.doi.org/10.1360/N032015-00122.

Y F Jiang , J McNeill . Chem Rev , 2017 . 117 ( 2 ): 838 - 859 . DOI:10.1021/acs.chemrev.6b00419http://doi.org/10.1021/acs.chemrev.6b00419.

T M Swager . Acc Chem Res , 1998 . 31 ( 5 ): 201 - 207.

S W Thomas , G D Joly , T M Swager . Chem Rev , 2007 . 107 ( 4 ): 1339 - 1386 . DOI:10.1021/cr0501339http://doi.org/10.1021/cr0501339.

D L Dexter . J Chem Phys , 1953 . 21 836 - 850 . DOI:10.1063/1.1699044http://doi.org/10.1063/1.1699044.

T Forster . Ann Phys , 1948 . 2 55 - 58.

R Y Zhan , B Liu . Macromol Chem Phys , 2015 . 216 ( 2 ): 131 - 144.

S Rochat , T M Swager . ACS Appl Mater Interfaces , 2013 . 5 ( 11 ): 4488 - 4502 . DOI:10.1021/am400939whttp://doi.org/10.1021/am400939w.

J W Wang , F T Lv , L B Liu , Y G Ma , S Wang . Coord Chem Rev , 2018 . 354 135 - 154 . DOI:10.1016/j.ccr.2017.06.023http://doi.org/10.1016/j.ccr.2017.06.023.

A J Heeger . Angew Chem Int Ed , 2001 . 40 ( 14 ): 2591 - 2611 . DOI:10.1002/(ISSN)1521-3773http://doi.org/10.1002/(ISSN)1521-3773.

B Liu , S Wang , G C Bazan . J Am Chem Soc , 2003 . 125 ( 44 ): 13306 - 13307 . DOI:10.1021/ja0365072http://doi.org/10.1021/ja0365072.

L H Feng , C L Zhu , H X Yuan , L B Liu , S Wang . Chem Soc Rev , 2013 . 42 6620 - 6633 . DOI:10.1039/c3cs60036jhttp://doi.org/10.1039/c3cs60036j.

L G Xu , L Cheng , C Wang , R Peng , Z Liu . Polym Chem , 2014 . 5 ( 5 ): 1573 - 1580 . DOI:10.1039/C3PY01196Hhttp://doi.org/10.1039/C3PY01196H.

H T Bai , H X Yuan , C Y Nie , B Wang , F T Lv , L B Liu , S Wang . Angew Chem Int Ed , 2015 . 54 ( 45 ): 13208 - 13213 . http://www.ncbi.nlm.nih.gov/pubmed/26307170.

S B Baylin , J E Ohm . Nat Rev Cancer , 2006 . 6 ( 2 ): 107 - 116 . DOI:10.1038/nrc1799http://doi.org/10.1038/nrc1799.

T J R Harris , F McCormick . Nat Rev Clin Oncol , 2010 . 7 251 - 265 . DOI:10.1038/nrclinonc.2010.41http://doi.org/10.1038/nrclinonc.2010.41.

F Sanger , S Nicklen , A R Coulson . Proc Natl Acad Sci , 1977 . 74 ( 12 ): 104 - 108.

M Ronaghi , M Uhlén , P Nyrén . Science , 1998 . 281 ( 5375 ): 363 - 365 . DOI:10.1126/science.281.5375.363http://doi.org/10.1126/science.281.5375.363.

Y Samuels , Z H Wang , A Bardelli , N Silliman , J Ptak , S Szabo , H Yan , A Gazdar , S M Powell , G J Riggins , J K V Willson , S Markowitz , K W Kinzler , B Vogelstein , V E Velculescu . Science , 2004 . 304 ( 5670 ): 554 DOI:10.1126/science.1096502http://doi.org/10.1126/science.1096502.

C T Wittwer , G H Reed , C N Gundry , J G Vandersteen , R J Pryor . Clin Chem , 2003 . 49 ( 6 ): 853 - 860 . DOI:10.1373/49.6.853http://doi.org/10.1373/49.6.853.

M Inazuka , T Tahira , K Hayashi . Genome Res , 1996 . 6 ( 6 ): 551 - 557 . DOI:10.1101/gr.6.6.551http://doi.org/10.1101/gr.6.6.551.

R E Board , N J Thelwell , P F Ravetto , S Little , M Ranson , C Dive , A Hughes , D Whitcombe . Clin Chem , 2008 . 54 ( 4 ): 757 - 760 . DOI:10.1373/clinchem.2007.098376http://doi.org/10.1373/clinchem.2007.098376.

P M Holland , R D Abramson , R Watson , D H Gelfand . Proc Natl Acad Sci , 1991 . 88 ( 16 ): 7276 - 7280 . DOI:10.1073/pnas.88.16.7276http://doi.org/10.1073/pnas.88.16.7276.

C D Hurst , T C M Zuiverloon , C Hafner , E C Zwarthoff , M A Knowles . BMC Res Notes , 2009 . 2 ( 1 ): 66 DOI:10.1186/1756-0500-2-66http://doi.org/10.1186/1756-0500-2-66.

J Z Song , Q Yang , F T Lv , L B Liu , S Wang . ACS Appl Mater Interfaces , 2012 . 4 ( 6 ): 2885 - 2890 . DOI:10.1021/am300830rhttp://doi.org/10.1021/am300830r.

X Y Wang , F He , F Tang , L D Li . J Mater Chem , 2012 . 22 ( 30 ): 15303 - 15308 . DOI:10.1039/c2jm32534ahttp://doi.org/10.1039/c2jm32534a.

J Z Song , J Y Zhang , F T Lv , Y Q Cheng , B Wang , L H Feng , L B Liu , S Wang . Angew Chem Int Ed , 2013 . 52 ( 49 ): 13020 - 13023 . http://www.ncbi.nlm.nih.gov/pubmed/24127406.

M Ehrlich . Oncogene , 2002 . 21 ( 35 ): 5400 - 5413 . DOI:10.1038/sj.onc.1205651http://doi.org/10.1038/sj.onc.1205651.

M Esteller , J G Herman . J Pathol , 2002 . 196 ( 1 ): 1 - 7 . DOI:10.1002/(ISSN)1096-9896http://doi.org/10.1002/(ISSN)1096-9896.

K D Robertson . Oncogene , 2001 . 20 ( 24 ): 3139 - 3155 . DOI:10.1038/sj.onc.1204341http://doi.org/10.1038/sj.onc.1204341.

Q Yang , Y Dong , W Wu , C L Zhu , H Chong , J Y Lu , D H Yu , L B Liu , F T Lv , S Wang . Nat Commun , 2012 . 3 1206 http://www.ncbi.nlm.nih.gov/pubmed/23149750.

J Y Zhang , B L Xing , J Z Song , C Y Nie , L Jiao , L B Liu , F T Lv , S Wang . Anal Chem , 2014 . 86 ( 1 ): 346 - 350 . DOI:10.1021/ac402720ghttp://doi.org/10.1021/ac402720g.

F D Feng , L B Liu , Q Yang , S Wang . Macromol Rapid Commun , 2010 . 31 ( 16 ): 1405 - 1421 . DOI:10.1002/marc.201000020http://doi.org/10.1002/marc.201000020.

Xingfen Liu , Xiaohui Cai , Yanqin Huang , Lin Shi , Quli Fan , Wei Huang . Acta Chimica Sinica , 2014 . 72 ( 4 ): 440 - 446 . http://www.cqvip.com/QK/91047X/201404/72888866504849524852484852.html.

刘兴奋 , 蔡小慧 , 黄艳琴 , 石琳 , 范曲立 , 黄维 . 化学学报 , 2014 . 72 ( 4 ): 440 - 446 . http://www.cqvip.com/QK/91047X/201404/72888866504849524852484852.html.

Pengfei Sun , Quli Fan , Lulin Liu , Weixing Deng , Xiaomei Lu , Wei Huang . Acta Polymerica Sinica , 2014 . ( 12 ): 1629 - 1634 . http://www.gfzxb.org/CN/abstract/abstract14198.shtml.

孙鹏飞 , 范曲立 , 柳露林 , 邓卫星 , 卢晓梅 , 黄维 .高分子学报 , 2014 . ( 12 ): 1629 - 1634 . http://www.gfzxb.org/CN/abstract/abstract14198.shtml.

X Y Wang , S L Li , P B Zhang , F T Lv , L B Liu , S Wang . Adv Mater , 2015 . 27 ( 39 ): 6040 - 6045 . DOI:10.1002/adma.201502880http://doi.org/10.1002/adma.201502880.

M Imaoka , S Yano , M Okumura , T Hibi , M Wakayama . Biosci Biotechnol Biochem , 2010 . 74 ( 9 ): 1936 - 1939 . http://www.ncbi.nlm.nih.gov/pubmed/20834145.

C J Ryan , W Peng , T Kheoh , E Welkowsky , C M Haqq , D W Chandler , H I Scher , A Molina . Prostate Cancer P D , 2014 . 172 ( 2 ): 192 - 198 . http://www.ncbi.nlm.nih.gov/pubmed/24637537.

Q S Wen , C L Zhu , L B Liu , Q Yang , S Wang , D B Zhu . Macromol Chem Phys , 2012 . 213 ( 23 ): 2486 - 2491 . DOI:10.1002/macp.v213.23http://doi.org/10.1002/macp.v213.23.

Xingfen Liu , Xiaoxiao Hua , Yanqin Huang , Xiaomiao Feng , Quli Fan , Wei Huang . Chemistry Bulletin , 2015 . 78 ( 10 ): 895 - 901 . http://www.cqvip.com/QK/94692X/201510/666353254.html.

刘兴奋 , 华笑笑 , 黄艳琴 , 冯晓苗 , 范曲立 , 黄维 . 化学通报 , 2015 . 78 ( 10 ): 895 - 901 . http://www.cqvip.com/QK/94692X/201510/666353254.html.

Xingfen Liu , Yateng Wang , Yanqin Huang , Xiaomiao Feng , Quli Fan , Wei Huang . Acta Chimica Sinica , 2016 . 74 ( 8 ): 664 - 668 . http://www.cqvip.com/QK/91047X/201608/669999622.html.

刘兴奋 , 王亚腾 , 黄艳琴 , 冯晓苗 , 范曲立 , 黄维 . 化学学报 , 2016 . 74 ( 8 ): 664 - 668 . http://www.cqvip.com/QK/91047X/201608/669999622.html.

P Gu , X F Liu , Y Y Tian , L Zhang , Q HuangY , S Su , X M Feng , Q L Fan , W Huang . Sensors Actuat B-Chem , 2017 . 246 78 - 84 . DOI:10.1016/j.snb.2017.01.043http://doi.org/10.1016/j.snb.2017.01.043.

O Lazcka , F J Del Campo , F X Munoz . Biosens Bioelectron , 2007 . 22 ( 7 ): 1205 - 1217 . DOI:10.1016/j.bios.2006.06.036http://doi.org/10.1016/j.bios.2006.06.036.

H X Yuan , Z Liu , L B Liu , F T Lv , Y L Wang , S Wang . Adv Mater , 2014 . 26 ( 25 ): 4333 - 4338 . DOI:10.1002/adma.v26.25http://doi.org/10.1002/adma.v26.25.

H T Bai , H Chen , R Hu , M Li , F T Lv , L B Liu , S Wang . ACS Appl Mater Interfaces , 2016 . 8 ( 46 ): 31550 - 31557 . DOI:10.1021/acsami.6b09807http://doi.org/10.1021/acsami.6b09807.

C Y Nie , S L Li , B Wang , L B Liu , R Hu , H Chen , F T Lv , Z Dai , S Wang . Adv Mater , 2016 . 28 ( 19 ): 3749 - 3754 . DOI:10.1002/adma.201600106http://doi.org/10.1002/adma.201600106.

Y Q Huang , X Yao , R Zhang , L Ouyang , R C Jiang , X F Liu , C X Song , G W Zhang , Q L Fan , L H Wang , W Huang . ACS Appl Mater Interfaces , 2014 . 6 ( 21 ): 19144 - 19153 . DOI:10.1021/am505113phttp://doi.org/10.1021/am505113p.

C Y Nie , B Wang , J Y Zhang , Y Q Cheng , F T Lv , L B Liu , S Wang . Small , 2015 . 11 ( 21 ): 2555 - 2563 . DOI:10.1002/smll.v11.21http://doi.org/10.1002/smll.v11.21.

R H Liu , Q L Cui , C Wang , X Y Wang , Y Yu , L D Li . ACS Appl Mater Interfaces , 2017 . 9 ( 3 ): 3006 - 3015 . DOI:10.1021/acsami.6b14320http://doi.org/10.1021/acsami.6b14320.

J Z Song , F T Lv , G M Yang , L B Liu , Q Yang , S Wang . Chem Commun , 2012 . 48 ( 60 ): 7465 - 7467 . DOI:10.1039/c2cc32085ahttp://doi.org/10.1039/c2cc32085a.

L H Feng , L B Liu , F T Lv , G C Bazan , S Wang . Adv Mater , 2014 . 26 ( 23 ): 3926 - 3930 . DOI:10.1002/adma.201305206http://doi.org/10.1002/adma.201305206.

B H Xia , X Y Wang , F He , Q L Cui , L D Li . ACS Appl Mater Interfaces , 2012 . 4 ( 11 ): 6332 - 6337 . DOI:10.1021/am301945khttp://doi.org/10.1021/am301945k.

B Wang , C L Zhu , L B Liu , F T Lv , Q Yang , S Wang . Polym Chem , 2013 . 4 ( 20 ): 5212 - 5215 . DOI:10.1039/c3py00097dhttp://doi.org/10.1039/c3py00097d.

Q L Cui , X Y Wang , Y Yang , S L Li , L D Li , S Wang . Chem Mater , 2016 . 28 ( 13 ): 4661 - 4669 . DOI:10.1021/acs.chemmater.6b01424http://doi.org/10.1021/acs.chemmater.6b01424.

F Y Wang , Z Liu , B Wang , L H Feng , L B Liu , F T Lv , Y L Wang , S Wang . Angew Chem , 2014 . 126 ( 2 ): 434 - 438 . DOI:10.1002/ange.v126.2http://doi.org/10.1002/ange.v126.2.

Y X Wang , S L Li , L H Feng , C Y Nie , L B Liu , F T Lv , S Wang . ACS Appl Mater Interfaces , 2015 . 7 ( 43 ): 24110 - 24118 . DOI:10.1021/acsami.5b07172http://doi.org/10.1021/acsami.5b07172.

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