缓冲液的组成和浓度对带正电荷纳米粒子<italic style="font-style: italic"><bold>ζ</bold></italic>电位的影响

黄韵晓; 阎虎生

doi:10.11777/j.issn1000-3304.2018.18007

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缓冲液的组成和浓度对带正电荷纳米粒子ζ电位的影响

研究论文 | 更新时间：2021-01-26

- 缓冲液的组成和浓度对带正电荷纳米粒子ζ电位的影响
- Effect of Buffer Types and Their Concentrations on ζ Potentials of Positively Charged Nanoparticles
- 高分子学报 2018年0卷第7期页码：893-899
- 作者机构：
  
  1.南开大学化学学院功能高分子材料教育部重点实验室　天津 300071
  2.天津化学化工协同创新中心　天津 300071
- 作者简介：
  
  E-mail: yanhs@nankai.edu.cn Hu-sheng Yan, E-mail: yanhs@nankai.edu.cn
- 基金信息：
  
  国家自然科学基金(基金号 51373080)资助项目()
- DOI：10.11777/j.issn1000-3304.2018.18007
  中图分类号：
- 纸质出版日期：2018-7，
  
  收稿日期：2018-1-8，
  
  修回日期：2018-3-29，
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黄韵晓, 阎虎生. 缓冲液的组成和浓度对带正电荷纳米粒子ζ电位的影响[J]. 高分子学报, 2018,0(7):893-899.

Yun-xiao Huang, Hu-sheng Yan. Effect of Buffer Types and Their Concentrations on ζ Potentials of Positively Charged Nanoparticles[J]. Acta Polymerica Sinica, 2018,0(7):893-899.
黄韵晓, 阎虎生. 缓冲液的组成和浓度对带正电荷纳米粒子ζ电位的影响[J]. 高分子学报, 2018,0(7):893-899. DOI： 10.11777/j.issn1000-3304.2018.18007.

Yun-xiao Huang, Hu-sheng Yan. Effect of Buffer Types and Their Concentrations on ζ Potentials of Positively Charged Nanoparticles[J]. Acta Polymerica Sinica, 2018,0(7):893-899. DOI： 10.11777/j.issn1000-3304.2018.18007.

摘要

研究了测定介质缓冲液的组成和浓度对3种含氨基纳米粒子的

电位的影响，这3种纳米粒子是聚酰胺-胺树状大分子(PAMAM)、苯乙烯与甲基丙烯酸-

-二甲基氨基乙基酯二嵌段共聚物(PS-

-PDMAEMA)胶束和壳聚糖纳米粒子. 在相同的pH值下，3种纳米粒子在三(羟甲基)氨基甲烷盐酸盐(Tris)、

-(2-羟乙基)哌嗪-

′-2-乙烷磺酸(Hepes)、3-(

-吗啡啉)丙磺酸(Mops)和磷酸盐缓冲液中的

电位依次降低，在磷酸盐缓冲液中的

电位比在其他几种缓冲液中的都低很多. 带有氨基的壳聚糖纳米粒子在浓度

~10 mmol/L磷酸盐缓冲液(pH = 7.4)中的

电位甚至是负值. 随着缓冲剂浓度的增加(从2 mmol/L到100 mmol/L) 3种纳米粒子的

电位都显著降低，在磷酸盐缓冲液(pH = 7.4)中随着缓冲剂浓度的增加，壳聚糖纳米粒子的

电位由正转负，转变点的磷酸盐的浓度为 ~ 10 mmol/L. 这一由正转负的现象可以归因于壳聚糖中氨基(p

= 6.3)在pH = 7.4下的低的质子化作用和存在三价的磷酸根负离子，三价磷酸根负离子牢固地结合于带正电荷的纳米粒子上，掩蔽了粒子的正电荷. 还研究了不同缓冲液的pH值和离子强度对

电位的影响.

Abstract

The application of charged nanoparticles in drug delivery and imaging has been extensively investigated. The surface charge density of charged nanoparticles

which is usually characterized by

potentials

has a drastic effect on the interaction between the nanoparticles and the biological systems

and this interaction is critical for the

in vivo

biofate of the nanoparticles. However

the effect of different types of buffer systems and their concentrations on the

potentials is often ignored in literature. Various buffer systems

such as phosphate

Tris

Hepes and Mops

and different buffer concentrations from 1 mmol/L to 200 mmol/L

were used for measuring

potentials of nanoparticles. Herein the effect of buffer types and their concentrations on the

potentials for three different types of amine group-containing nanoparticles

i.e.

poly(amido amine) (PAMAM) dendrimer

polystyrene-block-poly((

-diethylamino)ethyl methacrylate) (PS-

-PDMAEMA) micelles and chitosan nanoparticles

was studied. The

potentials of all the three types of nanoparticles decreased in the order of Tris

Hepes

Mops

and phosphates for buffer systems at the same concentration and pH. The

potentials were much lower in phosphate buffer than that in the others. The

potentials of amino group-containing chitosan nanoparticles showed even negative values in phosphate buffer with the concentration below ~ 10 mmol/L at pH = 7.4. The

potentials of all the three types of nanoparticles drastically decreased with the increase in buffer concentration (from 2 mmol/L to 100 mmol/L) for all the buffer systems investigated. The

potentials of chitosan nanoparticles in phosphate buffer (pH = 7.4) were reversed from positive to negative with the increase in phosphate concentration

with the crossover concentration of around 10 mmol/L. This charge reversal was contributed to the low protonation degree of chitosan amino groups (p

= 6.3) at pH = 7.4

and the presence of trivalent phosphate anions

which should be strongly adsorbed onto the positively charged particle surfaces and subsequently shielded the positive charges. When NaCl was added in the buffers

the

potentials of all the three types of nanoparticles decreased with increasing concentrations of the salt.

关键词

生物材料药物传输ζ电位带正电荷纳米粒子

Keywords

BiomaterialsDrug deliveryζ PotentialPositively charged nanoparticles

references

Wicki A, Witzigmann D, Balasubramanian V, Huwyler J . J Control Release , . 2015 . 200 138 - 157.

Pearson R M, Hsu H J, Bugno J, Hong S . MRS Bull , . 2014 . 39 227 - 237.

Fleischer C C, Payne C K . Acc Chem Res , . 2014 . 47 2651 - 2659.

Duncan R . Nat Rev Cancer , . 2006 . 6 688 - 701.

Fröhlich E . Int J Nanomed , . 2012 . 7 5577 - 5591.

Park J, Nam J, Won N, Jin H, Jung S, Jung S, Cho S H, Kim S . Adv Funct Mater , . 2011 . 21 1558 - 1566.

Amoozgar Z, Park J, Lin Q, Yeo Y . Mol Pharmaceut , . 2012 . 9 1262 - 1270.

Roser M, Fischer D, Kissel T . Eur J Pharm Biopharm , . 1998 . 46 255 - 263.

Lundqvist M, Stigler J, Elia G, Lynch I, Cedervall T, Dawson K A . Proc Natl Acad Sci USA , . 2008 . 105 14265 - 14270.

Ju C, Mo R, Xue J, Zhang L, Zhao Z, Xue L, Ping Q, Zhang C . Angew Chem Int Ed , . 2014 . 53 6253 - 6258.

Guo J, Ping Q, Jiang G, Huang L, Tong Y . Int J Pharm , . 2003 . 260 167 - 173.

Zhou Z, Ma X, Murphy C J, Jin E, Sun Q, Shen Y, Van Kirk E A, Murdoch W J . Angew Chem Int Ed , . 2014 . 53 10949 - 10955.

Oh N M, Oh K T, Lee E S . Colloid Surface B , . 2014 . 19 14 - 21.

Wu W, Wang J, Lin Z, Li X, Li J . Macromol Rapid Commun , . 2014 . 35 1679 - 1684.

Kaur R, Henriksen-Lacey M, Wilkhu J, Devitt A, Christensen D, Perrie Y . Mol Pharmaceut , . 2014 . 11 197 - 207.

Qian R, Ding L, Ju H . J Am Chem Soc , . 2013 . 135 13282 - 13285.

Sarker S R, Hokama R, Takeoka S . Mol Pharmaceut , . 2014 . 11 164 - 174.

Zhu C, Zheng M, Meng F, Mickler F M, Ruthardt N, Zhu X, Zhong Z . Biomacromolecules , . 2012 . 13 769 - 778.

Tang M X, Szoka F C . Gene Therapy , . 1997 . 4 823 - 832.

Ladewig K, Xu Z P, Gray P, Lu G Q . J Biomed Mater Res Part A , . 2014 . 102 2137 - 2146.

Zhang S, Zhou K, Huang G, Takahashi M, Sherry A D, Gao J . Chem Commun , . 2013 . 49 6418 - 6420.

Tseng S J, Liao Z X, Kao S H, Zeng Y F, Huang K Y, Li H J, Yang C L, Deng Y F, Huang C F, Yang S C, Yang P C, Kempson I M . Nat Commun , . 2015 . 6 6456 .

Li L, Raghupathi K, Yuan C, Thayumanavan S . Chem Sci , . 2013 . 4 3654 - 3660.

Bigall N C, Curcio A, Leal M P, Falqui A, Palumberi D, Corato R D, Albanesi E, Cingolani R, Pellegrino T . Adv Mater , . 2011 . 23 5645 - 5650.

Liu Q, Yin B, Yang T, Yang Y, Shen Z, Yao P, Li F . J Am Chem Soc , . 2013 . 135 5029 - 5037.

Mahmoud K A, Mena J A, Male K B, Hrapovic S, Kamen A, Luong J H T . ACS Appl Mater Interfaces , . 2010 . 2 2924 - 2932.

Mostaghaci B, Loretz B, Haberkorn R, Kickelbick G, Lehr C M . Chem Mater , . 2013 . 25 3667 - 3674.

Park J, Nam J, Won N, Jin H, Jung S, Jung S, Cho S H, Kim S . Adv Funct Mater , . 2011 . 21 1558 - 1566.

Guo L, Wang C, Yang C, Wang X, Zhang T, Zhang Z, Yan H, Liu K . Polymer , . 2016 . 84 189 - 197.

Chorom M, Rengasamy P . Eur J Soil Sci , . 1995 . 46 657 - 665.

Tomalia D A, Naylor A M, Goddard W A . Angew Chem Int Ed , . 1990 . 29 138 - 175.

Su Y L, Li C . J Colloid Interf Sci , . 2007 . 316 344 - 349.

Bhattarai N, Gunn J, Zhang M . Adv Drug Deliv Rev , . 2010 . 62 83 - 99.

Du J Z, Sun T M, Song W J, Wu J, Wang J . Angew Chem Int Ed , . 2010 . 49 3621 - 3626.

Zhang Y, Wang C, Xu C, Yang C, Zhang Z, Yan H, Liu K . Chem Commun , . 2013 . 49 7286 - 7288.

Chen W, Achazi K, Schade B, Haag R . J Control Release , . 2015 . 205 15 - 24.

Lu J, Jia H, Guo L, Zhang G, Cao Y, Yan H, Liu K . Eur Polym J , . 2015 . 66 376 - 385.

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缓冲液的组成和浓度对带正电荷纳米粒子ζ电位的影响

Effect of Buffer Types and Their Concentrations on ζ Potentials of Positively Charged Nanoparticles

DOI：10.11777/j.issn1000-3304.2018.18007

摘要

Abstract

关键词

Keywords

references