基于氢键力学增强型维生素D<sub>3</sub>脂质体复合水凝胶的构建及骨修复研究

程若昱; 崔文国

doi:10.11777/j.issn1000-3304.2018.18066

您当前的位置：

首页 >

文章列表页 >

基于氢键力学增强型维生素D₃脂质体复合水凝胶的构建及骨修复研究

研究论文 | 更新时间：2021-01-26

- 基于氢键力学增强型维生素D₃脂质体复合水凝胶的构建及骨修复研究
- Hydrogen Bond Mechanically Enhanced Cholecalciferol Lipo-Hydrogel for Bone Regeneration
- 高分子学报 2018年0卷第10期页码：1315-1327
- 作者机构：
  
  1.苏州大学医学部骨科研究所　苏州 215006
  2.上海市中西医结合防治骨与关节病损重点实验室上海市伤骨科研究所上海交通大学医学院附属瑞金医院　上海 200025
  3.聚合物分子工程国家重点实验室复旦大学高分子科学系　上海 200433
- 作者简介：
  
  E-mail: wgcui80@hotmail.com
- 基金信息：
  
  国家自然科学基金(基金号 51373112)资助项目()
- DOI：10.11777/j.issn1000-3304.2018.18066
  中图分类号：
- 纸质出版日期：2018-10，
  
  收稿日期：2018-2-26，
  
  修回日期：2018-3-27，
扫描看全文
程若昱, 崔文国. 基于氢键力学增强型维生素D₃脂质体复合水凝胶的构建及骨修复研究[J]. 高分子学报, 2018,0(10):1315-1327.

Ruo-yu Cheng, Wen-guo Cui. Hydrogen Bond Mechanically Enhanced Cholecalciferol Lipo-Hydrogel for Bone Regeneration[J]. Acta Polymerica Sinica, 2018,0(10):1315-1327.
程若昱, 崔文国. 基于氢键力学增强型维生素D₃脂质体复合水凝胶的构建及骨修复研究[J]. 高分子学报, 2018,0(10):1315-1327. DOI： 10.11777/j.issn1000-3304.2018.18066.

Ruo-yu Cheng, Wen-guo Cui. Hydrogen Bond Mechanically Enhanced Cholecalciferol Lipo-Hydrogel for Bone Regeneration[J]. Acta Polymerica Sinica, 2018,0(10):1315-1327. DOI： 10.11777/j.issn1000-3304.2018.18066.

摘要

基于氢键和光交联双交联网络构建力学增强型维生素D

脂质体复合水凝胶，并通过药物控释促进成骨分化. 通过甲基丙烯酸酯明胶(GelMA)与载药脂质体的氢键作用，形成双交联网络，实现复合水凝胶体系最大断裂力值和弹性模量均提升至原来的2倍. 通过脂质体对于脂溶性药物维生素D

的包载，实现维生素D

在水凝胶网络中均匀分散和缓慢释放. 体外研究表明，该复合水凝胶比GelMA有着更显著的促进成骨分化作用，且不影响细胞的增殖黏附. 因此，该类型脂质体增强型水凝胶在拓宽水凝胶装载和控释药物及组织修复方面有良好的前景.

Abstract

Hydrogel is an excellent candidate for drug delivery and tissue regeneration. However

hydrogel used as drug carriers is facing some problems

including limited optional drug and burst drug release. In addition

hydrogel is still confronting some challenges in the area of mechanical property. Cholecalciferol is a type of vitamin able to promote ossification. However

as a lipophilic drug

it should be taken according to the recommended dosage. That means the carriers must be capable of making hydrophobic drug homodispersed with controlling drug release. In this study

we designed a novel hydrogen bond based mechanical reinforced hydrogel

modified by release-controlling cholecalciferol liposome

as an multifunctional scaffold for promoting osteogenesis during the process of bone regeneration. Specifically

drug-loaded liposome was fabricated and the characteristics of the liposomes were investigated. Further

photo-crosslinkable gelatin derivative (GelMA) was combined with drug loaded liposome by micro-cross linking double network

generated by the interaction between hydrogen bond and hydrogel network

leading to double enhancement in mechanical property both for compression and streching. Furthermore

the ability to control release and support uniform distribution of hydrophobic drug (cholecalciferol) was observed in the composite hydrogel

specifically the sustained release of cholecalciferol lasted for three weeks at least without significant brust release.

In vitro

study also revealed that

this composite hydrogel was proved to have excellent biocompatibility without significant influence on MC3T3-E1 cell adhesion

proliferation

compared with GelMA hydrogel and control groups. After 21 days cultivation

MC3T3-E1 cells cultured with this composite hydrogel exhibited remarkably facilitated osteogenic differentiation

according to the result of ALP stain

ALP analysis and Alizarin red staining. Thus

it is reasonable to believe that this liposome composite hydrogel could provide a promising strategy for extending the application of hydrogel in drug delivery and tissue reconstruction.

关键词

脂质体水凝胶控制释放力学增强骨修复

Keywords

LiposomeHydrogelControl releaseMechanical enhancementBone regeneration

references

Peppas N A, Hilt J Z, Khademhosseini A, Langer R . Adv Mater , 2006 . 18 ( 11 ): 1345 - 1360 . DOI:10.1002/(ISSN)1521-4095http://doi.org/10.1002/(ISSN)1521-4095 .

Ganji F, Vasheghani-Farahani S, Vasheghani-Farahani E . Iran Polym J , 2010 . 19 ( 5 ): 375 - 398.

Billiet T, Vandenhaute M, Schelfhout J, Van V S, Dubruel P . Biomaterials , 2012 . 33 ( 26 ): 6020 - 6041 . DOI:10.1016/j.biomaterials.2012.04.050http://doi.org/10.1016/j.biomaterials.2012.04.050 .

Vlierberghe SV, Dubruel P, Schacht E . Biomacromolecules , 2011 . 12 ( 5 ): 1387 - 1408 . DOI:10.1021/bm200083nhttp://doi.org/10.1021/bm200083n .

Tsai E C, Dalton P D, Shoichet M S, Tator C H . Biomaterials , 2006 . 27 ( 3 ): 519 - 533 . DOI:10.1016/j.biomaterials.2005.07.025http://doi.org/10.1016/j.biomaterials.2005.07.025 .

Patterson J, Siew R, Herring S W, Lin A S P, Guldberg R, Stayton P S . Biomaterials , 2010 . 31 ( 26 ): 6772 - 6781 . DOI:10.1016/j.biomaterials.2010.05.047http://doi.org/10.1016/j.biomaterials.2010.05.047 .

Hori K, Sotozono C, Hamuro J, Yamasaki K, Kimura Y, Ozeki M, Tabata Y, Kinoshita S . J Control Release , 2007 . 118 ( 1 ): 169 - 176.

Yu Y, Wang Y, Feng C . Chem Res Chinese Univ , 2016 . 32 ( 5 ): 872 - 876 . DOI:10.1007/s40242-016-5474-2http://doi.org/10.1007/s40242-016-5474-2 .

Andrésguerrero V, Zong M, Ramsay E, Rojas B, Sarkhel S, Gallego B, de H R, Ramírez A I, Salazar J J, Triviño A, Ramírez J M, Del Amo E M, Cameron N, de-Las-Heras B, Urtti A, Mihov G, Dias A, Herrero-Vanrell R . J Control Release , 2015 . 10 ( 211 ): 105 - 107.

Nguyen V N, Huang N, Grossiord J L, Moine L, Agnely F, Vauthier C . J Appl Polym Sci , 2013 . 128 ( 5 ): 3113 - 3121 . DOI:10.1002/app.v128.5http://doi.org/10.1002/app.v128.5 .

Barenholz Y . Curr Opin Colloid Interface Sci , 2001 . 6 ( 1 ): 66 - 77 . DOI:10.1016/S1359-0294(00)00090-Xhttp://doi.org/10.1016/S1359-0294(00)00090-X .

Teschke O, Souza E F D . Langmuir , 2002 . 18 ( 18 ): 6513 - 6520.

Mourtas S, Fotopoulou S, Duraj S, Sfika V, Tsakiroglou C, Antimisiaris S G . Colloid Surface B , 2007 . 55 ( 2 ): 212 - 221 . DOI:10.1016/j.colsurfb.2006.12.005http://doi.org/10.1016/j.colsurfb.2006.12.005 .

Bhardwaj U, Burgess D J . Int J Pharm , 2010 . 388 ( 1-2 ): 181 - 189 . DOI:10.1016/j.ijpharm.2010.01.003http://doi.org/10.1016/j.ijpharm.2010.01.003 .

Cha C, Shin SR, Gao X, Annabi N, Dokmeci M R, Tang X S, Khademhosseini A . Small , 2014 . 10 ( 3 ): 514 - 523 . DOI:10.1002/smll.v10.3http://doi.org/10.1002/smll.v10.3 .

Zhang Y, Gao Z, Yu Z, Ren X Y, Duan L J, Gao G H . New J Chem , 2017 . 41 ( 4 ): 3281 - 3295.

Lyu D, Chen S, Guo W . Small , 2018 . 14 39 - 46.

Yue K, Trujillode S G, Alvarez M M, Tamayol A, Annabi N, Khademhosseini A . Biomaterials , 2015 . 73 254 - 271 . DOI:10.1016/j.biomaterials.2015.08.045http://doi.org/10.1016/j.biomaterials.2015.08.045 .

Chen P, Xia C, Mei S, Wang J, Shan Z, Lin X, Fan S . Biomaterials , 2016 . 81 1 - 13 . DOI:10.1016/j.biomaterials.2015.12.006http://doi.org/10.1016/j.biomaterials.2015.12.006 .

Chen H, Guo L, Wicks J, Ling C, Zhao X, Yan Y, Qi J, Cui W, Deng L . J Mater Chem B , 2016 . 4 ( 21 ): 3770 - 3781 . DOI:10.1039/C6TB00065Ghttp://doi.org/10.1039/C6TB00065G .

Wu W, Ni Q, Xiang Y, Dai Y, Jiang S, Wan L, Liu X, Cui W . RSC Adv , 2016 . 6 ( 95 ): 92449 - 92453.

Li P, Dou X, Feng C, Schoenherr H . Biomater Sci , 2018 . 6 ( 4 ): 785 - 792 . DOI:10.1039/C7BM00991Ghttp://doi.org/10.1039/C7BM00991G .

Hurler J, S Åa, Mravljak J, Pajk S, Kristl A, Schubert R, Škalko-Basnet N . Int J Pharm , 2013 . 450 ( 1 ): 296 - 303.

Cheng H, Yue K, Kazemzadeh-Narbat M, Liu Y, Khalilpour A, Li B, Zhang Y S, Annabi N, Khademhosseini A . ACS Appl Mater Interfaces , 2017 . 9 ( 13 ): 11428 DOI:10.1021/acsami.6b16779http://doi.org/10.1021/acsami.6b16779 .

Shin H, Olsen B D, Khademhosseini A . Biomaterials , 2012 . 33 ( 11 ): 3143 - 3152 . DOI:10.1016/j.biomaterials.2011.12.050http://doi.org/10.1016/j.biomaterials.2011.12.050 .

Suri A, Rui C, Rackus D G, Spiller N J S, Richardson C, Pålsson L O, Kataky R . Soft Matter , 2011 . 7 ( 15 ): 7071 - 7077 . DOI:10.1039/c1sm05530ehttp://doi.org/10.1039/c1sm05530e .

Xin T, Gu Y, Cheng R, Tang J, Sun Z, Cui W, Chen L . ACS Appl Mater Interfaces , 2017 . 9 ( 47 ): 41168 - 41180 . DOI:10.1021/acsami.7b13167http://doi.org/10.1021/acsami.7b13167 .

Bartnikowski M, Bartnikowski NJ, Woodruff MA, Schrobback K, Klein TJ . Acta Biomater , 2015 . 27 66 - 76 . DOI:10.1016/j.actbio.2015.08.038http://doi.org/10.1016/j.actbio.2015.08.038 .

. 高分子学报 , 2012 . ( 10 ): 1143 - 1150 . DOI:10.3724/SP.J.1105.2012.12129http://doi.org/10.3724/SP.J.1105.2012.12129 .

Ye Chen(叶晨), Li Zhenhua(李振华), Li Dan(李丹), Gao Changyou(高长友) . Acta Polymerica Sinica , 2012 . ( 10 ): 1143 - 1150 . DOI:10.3724/SP.J.1105.2012.12129http://doi.org/10.3724/SP.J.1105.2012.12129 .

. 高分子学报 , 2012 . ( 7 ): 778 - 783 . DOI:10.3724/SP.J.1105.2012.12013http://doi.org/10.3724/SP.J.1105.2012.12013 .

Li Cong(李聪), Zhang Zhe(张喆), He Chaoliang(贺超良), Zhuang Xiuli(庄秀丽), Chen Li(陈莉), Cai Qing(蔡青), Xue Yan(薛妍), Zhang Bin(张宾), Wang Shizhen(王世振), Meng Weiyan(孟维艳), Chen Xuesi(陈学思) . Acta Polymerica Sinica , 2012 . ( 7 ): 778 - 783 . DOI:10.3724/SP.J.1105.2012.12013http://doi.org/10.3724/SP.J.1105.2012.12013 .

Rahaman M N, Day D E, Bal B S, Fu Q, Jung S B, Bonewald LF, Tomsia A P . Acta Biomater , 2011 . 7 ( 6 ): 2355 - 2373 . DOI:10.1016/j.actbio.2011.03.016http://doi.org/10.1016/j.actbio.2011.03.016 .

更多指标>

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

聚硫辛酸基多功能水凝胶的构建及在感染皮肤伤口修复中的应用

膨润土基水凝胶黏合剂的可控构筑及其对砂岩质文物的长效保护应用研究

基于葫芦［8］脲主客体化学的双客体组分动态交联水凝胶的制备和性能研究

具有葡萄糖响应性释药的多糖基可注射自愈合水凝胶

利用高分子多基元协同效应增强脂质体稳定性的研究

基于氢键力学增强型维生素D3脂质体复合水凝胶的构建及骨修复研究

Hydrogen Bond Mechanically Enhanced Cholecalciferol Lipo-Hydrogel for Bone Regeneration

DOI：10.11777/j.issn1000-3304.2018.18066

摘要

Abstract

关键词

Keywords

references

基于氢键力学增强型维生素D₃脂质体复合水凝胶的构建及骨修复研究