基于桑蚕丝蛋白的多肽/两亲性多肽及其组装和功能化

陈红; 邵正中

doi:10.11777/j.issn1000-3304.2018.18083

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基于桑蚕丝蛋白的多肽/两亲性多肽及其组装和功能化

专论 | 更新时间：2021-01-26

- 基于桑蚕丝蛋白的多肽/两亲性多肽及其组装和功能化
- Peptides/Amphiphilic Peptides Based Silk Fibroin of Bombxy Mori and Their Self-assembly and Functionalization
- 高分子学报 2018年0卷第8期页码：987-996
- 作者机构：
  
  聚合物分子工程国家重点实验室复旦大学高分子科学系和先进材料实验室　上海 200438
- 作者简介：
  
  [ "邵正中，男，1964年出生. 1981年至1991年于复旦大学求学，获理学博士学位. 毕业后留校从事科研和教学工作至今(1996年至1998 年期间在丹麦Aarhus大学生物研究所工作)，1999年晋升为教授；教育部“长江学者奖励计划”特聘教授、国家杰出青年基金获得者、英国皇家化学会会士(Fellow of Royal Society of Chemistry，FRSC). 现兼任上海市化学化工学会理事、英国皇家化学会Journal of Materials Chemistry-B副主编、Chinese Journal of Polymer Science编委会成员及International Bionic Award评委会成员等职. 主要研究方向为生物大分子，着重从高分子科学的角度对结构性生物大分子如动物丝及其相应丝蛋白和几丁质及壳聚糖等的结构、性能和仿生制备等方面进行研究，以及其在结构性材料、生物医用材料和仿生矿化材料等领域中的多元化应用创造良好条件. 曾入选上海市青年科技启明星、教育部新世纪优秀人才和国家级“新世纪百千万人才工程”等；获教育部自然科学奖一等奖(第一获奖人)、上海市自然科学奖一等奖(第一获奖人)和上海市自然科学“牡丹奖”等多项奖励" ]
- 基金信息：
  
  国家自然科学基金(基金号 21574024)资助项目()
- DOI：10.11777/j.issn1000-3304.2018.18083
  中图分类号：
- 纸质出版日期：2018-8，
  
  收稿日期：2018-3-19，
  
  修回日期：2018-4-9，
扫描看全文
陈红, 邵正中. 基于桑蚕丝蛋白的多肽/两亲性多肽及其组装和功能化[J]. 高分子学报, 2018,0(8):987-996.

Hong Chen, Zheng-zhong Shao. Peptides/Amphiphilic Peptides Based Silk Fibroin of Bombxy Mori and Their Self-assembly and Functionalization[J]. Acta Polymerica Sinica, 2018,0(8):987-996.
陈红, 邵正中. 基于桑蚕丝蛋白的多肽/两亲性多肽及其组装和功能化[J]. 高分子学报, 2018,0(8):987-996. DOI： 10.11777/j.issn1000-3304.2018.18083.

Hong Chen, Zheng-zhong Shao. Peptides/Amphiphilic Peptides Based Silk Fibroin of Bombxy Mori and Their Self-assembly and Functionalization[J]. Acta Polymerica Sinica, 2018,0(8):987-996. DOI： 10.11777/j.issn1000-3304.2018.18083.

摘要

多肽的自组装因其独特性质和作用已成为超分子化学、高分子功能材料和生物医用材料等领域的研究热点. 桑蚕丝蛋白具有高度重复的多肽序列，其组装行为和相应的结构对动物丝及丝蛋白基材料的形成和特有的优异综合性能起到了至关重要的作用. 通过特定的酶降解桑蚕丝蛋白能够以较为经济的方式获得多种明确序列的多肽，并可进一步方便地制备功能性的两亲性多肽. 本文结合国内外多肽自组装的研究现状，对本课题组在桑蚕丝蛋白多肽/两亲性多肽的制备、组装以及功能化等方面的研究成果进行了总结，同时也展望了其在若干领域中的实际应用.

Abstract

Given the unique properties and functions

such as bioactivity and good biocompatibility

the self-assembly of peptide

specially amphipathic peptide

and their applications have become the research focus in the fields of supermolecular chemistry as well as functional polymeric and biomedical materials. Generally

most of peptide building blocks are prepared from chemical synthesis such as solid phase peptide synthesis

or genetic engineering. Those " bottom up” methods may be suffered by time consuming and cost

which limit the practical application of assembly peptides materials. Degradation of natural proteins such as casein

corn protein and so on

provides us a convenient way to obtain the mixed peptides. However

most of researches about these mixtures are focused on their bio-functions or surfactant property. The usage of them as building blocks of assembly peptide is overlooked. Silk fibroin from

Bombyx mori

silkworm silk is with highly repetitive sequences

such as GAGAGY and GAGAGS

their different assembly properties and the relevant structures not only play an important role in the forming of animal silks and silk fibroin based materials

but also have crucial effect on their excellent comprehensive performance. According to the selectivity of different enzymes

there are enzymes which work on the Tyr-Gly or Ala-Gly peptide bone

respectively. Therefore

it provides us the opportunity to harvest the silk peptides with special sequence economically

via

degrading silk fibroin by those enzymes. We introduced in this paper the enzymatic degradation

a " top down” method on obtaining peptide with specific sequences from silk fibroin and investigated their assembly properties. In addition

we summarized the construction of functionalization amphiphilic peptides with one of those silk peptides (GAGAGAGY) as building block as well as their assembly and applications like the forming pH responsive peptide hydrogel

flexible thermochromism materials and unique peptide surfactant. Finally

we prospected the application of top down method on obtaining functional peptide building blocks as well as application of the silk peptide obtained by this way on construction functional peptides.

关键词

天然蛋白质选择性降解氨基酸序列水凝胶响应性

Keywords

Natural proteinSelective degradationAmino acid sequencesHydrogelResponsive

references

Wang J, Liu K, Xing R, Yan X . Chem Soc Rev , . 2016 . 45 ( 20 ): 5589 - 5604 . DOI:10.1039/C6CS00176Ahttp://doi.org/10.1039/C6CS00176A .

Zhao X B, Pan F, Xu H, Yaseen M, Shan H H, Hauser C A E, Zhang S G, Lu J R . Chem Soc Rev , . 2010 . 39 ( 9 ): 3480 - 3498 . DOI:10.1039/b915923chttp://doi.org/10.1039/b915923c .

Boekhoven J, Stupp S I . Adv Mater , . 2014 . 26 ( 11 ): 1642 - 1659 . DOI:10.1002/adma.201304606http://doi.org/10.1002/adma.201304606 .

Qiu Wenxiu(邱文秀), Cheng Han(程翰), Zhang Xianzheng(张先正), Zhuo Renxi(卓仁禧) . 高分子学报 , . 2018 . ( 1 ): 32 - 44.

Wang Youzhi(王友志), Gao Jie(高洁), Yang Zhimou(杨志谋) . 高分子学报 , . 2018 . ( 1 ): 9 - 20.

Koh L D, Cheng Y, Teng C P, Khin Y W, Loh X J, Tee S Y, Low M, Ye E, Yu H D, Zhang Y W, Han M Y . Prog Polym Sci , . 2015 . 46 86 - 110 . DOI:10.1016/j.progpolymsci.2015.02.001http://doi.org/10.1016/j.progpolymsci.2015.02.001 .

Joseph B, Raj S J . Front Life Sci , . 2012 . 6 ( 3-4 ): 55 - 60 . DOI:10.1080/21553769.2012.760491http://doi.org/10.1080/21553769.2012.760491 .

Mita K, Ichimura S, James T C . J Mol Evol , . 1994 . 38 ( 6 ): 583 - 592.

Gong Z G, Huang L, Yang Y H, Chen X, Shao Z Z . Chem Commun , . 2009 . 48 ( 48 ): 7506 - 7508.

Hao Ruiwen(郝瑞文). Research on Preparation and Assembly Behavior of Silk Peptides(丝肽的制备及其组装行为研究). Doctoral Dissertation of Fudan University(复旦大学博士学位论文), 2010

Hao R, Zhang J, Xu T, Huang L, Yao J, Chen X, Shao Z . Polym Chem , . 2013 . 4 ( 10 ): 3005 - 3011 . DOI:10.1039/c3py21096khttp://doi.org/10.1039/c3py21096k .

Ling S J, Li C X, Adamcik J, Shao Z Z, Chen X, Mezzenga R . Adv Mater , . 2014 . 26 ( 26 ): 4569 - 4574 . DOI:10.1002/adma.v26.26http://doi.org/10.1002/adma.v26.26 .

Liu Y X, Ling S J, Wang S H, Chen X, Shao Z Z . Biomater Sci , . 2014 . 2 ( 10 ): 1338 - 1342 . DOI:10.1039/C4BM00214Hhttp://doi.org/10.1039/C4BM00214H .

Löwik D W, Garcia-Hartjes J, Meijer J T, van Hest J C . Langmuir , . 2005 . 21 ( 2 ): 524 - 526 . DOI:10.1021/la047578xhttp://doi.org/10.1021/la047578x .

Cui H, Webber M J, Stupp S I . Biopolymers , . 2010 . 94 ( 1 ): 1 - 18 . DOI:10.1002/bip.21328http://doi.org/10.1002/bip.21328 .

Pashuck E T, Cui H, Stupp S I . J Am Chem Soc , . 2010 . 132 ( 17 ): 6041 - 6046 . DOI:10.1021/ja908560nhttp://doi.org/10.1021/ja908560n .

Zhang J, Hao R, Huang L, Yao J, Chen X, Shao Z . Chem Commun , . 2011 . 47 ( 37 ): 10296 - 10298 . DOI:10.1039/c1cc12633dhttp://doi.org/10.1039/c1cc12633d .

Paramonov S E, Howook Jun A, Hartgerink J D . J Am Chem Soc , . 2006 . 128 ( 22 ): 7291 - 7298 . DOI:10.1021/ja060573xhttp://doi.org/10.1021/ja060573x .

Sadownik J W, Leckie J, Ulijn R V . Chem Commun , . 2011 . 47 ( 2 ): 728 - 730 . DOI:10.1039/C0CC03796Fhttp://doi.org/10.1039/C0CC03796F .

Ghosh A, Dobson E T, Buettner C J, Nicholl M J, Goldberger J E . Langmuir , . 2014 . 30 ( 51 ): 15383 - 15387 . DOI:10.1021/la5037663http://doi.org/10.1021/la5037663 .

Zhao F Y, Guo H, Zhang Z D, Ye J, Liu L L, Zhao C X, Shao Z Z . J Mater Chem B , . 2017 . 5 ( 26 ): 5189 - 5195 . DOI:10.1039/C7TB00736Ahttp://doi.org/10.1039/C7TB00736A .

Guo H, Zhang J M, Xu T, Zhang Z D, Yao J R, Shao Z Z . Biomacromolecules , . 2013 . 14 ( 8 ): 2733 - 2738 . DOI:10.1021/bm4005645http://doi.org/10.1021/bm4005645 .

Zhang S M, Greenfield M A, Mata A, Palmer L C, Bitton R, Mantei J R, Aparicio C, Cruz, M O, Stupp, S I . Nat Mater , . 2010 . 9 ( 7 ): 594 - 601 . DOI:10.1038/nmat2778http://doi.org/10.1038/nmat2778 .

Jelinek R, Ritenberg M . RSC Adv , . 2013 . 3 ( 44 ): 21192 - 21201 . DOI:10.1039/c3ra42639dhttp://doi.org/10.1039/c3ra42639d .

Diegelmann S R, Tovar J D . Macromol Rapid Commun , . 2013 .34 ( 17 ): 1343 - 1350 . DOI:10.1002/marc.v34.17http://doi.org/10.1002/marc.v34.17 .

Guo H, Zhang J M, Porter D, Peng H, Löwik D W P M, Wang Y, Zhang Z D, Chen X, Shao Z Z . Chem Sci , . 2014 . 5 ( 11 ): 4189 - 4195 . DOI:10.1039/C4SC01696Chttp://doi.org/10.1039/C4SC01696C .

Peng H S, Sun X M, Cai F J, Chen X L, Zhu Y C, Liao G P, Chen D Y, Li Q W, Lu Y F, Zhu Y T, Jia Q X . Nat Nano , . 2009 . 4 ( 11 ): 738 - 741 . DOI:10.1038/nnano.2009.264http://doi.org/10.1038/nnano.2009.264 .

Feng H B, Lu J, Li J H, Tsow F, Forzani E, Tao N J . Adv Mater , . 2013 . 25 ( 12 ): 1729 - 1733 . DOI:10.1002/adma.v25.12http://doi.org/10.1002/adma.v25.12 .

Lu X, Zhang Z D, Sun X M, Chen P N, Zhang J, Guo H, Shao Z Z, Peng H S . Chem Sci , . 2016 . 7 ( 8 ): 5113 - 5117 . DOI:10.1039/C6SC00414Hhttp://doi.org/10.1039/C6SC00414H .

Li Y, Tang Z, Prasad P N, Knecht M R, Swihart M T . Nanoscale , . 2014 . 6 ( 6 ): 3165 - 3172 . DOI:10.1039/C3NR06201Ehttp://doi.org/10.1039/C3NR06201E .

Munro C J, Hughes Z E, Walsh T R, Knecht M R . J Phys Chem C , . 2016 . 120 ( 33 ): 18917 - 18924 . DOI:10.1021/acs.jpcc.6b06046http://doi.org/10.1021/acs.jpcc.6b06046 .

Yu L, Banerjee I A, Shima M, Rajan K, Matsui H . Adv Mater , . 2004 . 16 ( 8 ): 709 - 712 . DOI:10.1002/(ISSN)1521-4095http://doi.org/10.1002/(ISSN)1521-4095 .

Tang Z, Kotov N A . Adv Mater , . 2005 . 36 ( 25 ): 951 - 962.

Zhang J M, Xu T, Yao J R, Huang L, Chen X, Shao Z Z . RSC Adv , . 2012 . 2 ( 13 ): 5599 - 5604 . DOI:10.1039/c2ra20259jhttp://doi.org/10.1039/c2ra20259j .

Merg A D, Boatz J C, Mandal A, Zhao G P, Mokashi-Punekar S, Liu C, Wang X T, Zhang P J, van der Wel, P C A, Rosi N L . J Am Chem Soc , . 2016 . 138 ( 41 ): 13655 - 13663 . DOI:10.1021/jacs.6b07322http://doi.org/10.1021/jacs.6b07322 .

Mokashi-Punekar S, Merg A D, Rosi N L . J Am Chem Soc , . 2017 . 139 ( 42 ): 15043 - 15048 . DOI:10.1021/jacs.7b07143http://doi.org/10.1021/jacs.7b07143 .

Cai Huanxin(蔡焕新), Yin Baoru(殷宝茹), Yao Ping(姚萍) . 色谱 , . 2010 . 28 ( 7 ): 637 - 643.

Zhou L, Xu G, Zhang Z, Li H, Yao P . Colloid Surface A , . 2018 . 540 150 - 157 . DOI:10.1016/j.colsurfa.2017.12.070http://doi.org/10.1016/j.colsurfa.2017.12.070 .

Feng Z, Wang H, Zhou R, Li J, Xu B . J Am Chem Soc , . 2017 . 139 ( 11 ): 3950 - 3953 . DOI:10.1021/jacs.7b00070http://doi.org/10.1021/jacs.7b00070 .

Sahoo J K, Pappas C G, Sasselli I R, Abul-Haija Y M, Ulijn R V . Angew Chem Int Ed , . 2017 . 56 ( 24 ): 6828 - 6832 . DOI:10.1002/anie.201701870http://doi.org/10.1002/anie.201701870 .

Wibowo D, Wang H F, Shao Z, Middelberg A P J, Zhao C X . J Phys Chem C , . 2017 . 121 ( 27 ): 14658 - 14657 . DOI:10.1021/acs.jpcc.7b03807http://doi.org/10.1021/acs.jpcc.7b03807 .

Wang H F, Wibowo D, Shao Z, Apj M, Zhao C X . Langmuir , . 2017 . 33 ( 32 ): 7957 - 7967 . DOI:10.1021/acs.langmuir.7b01382http://doi.org/10.1021/acs.langmuir.7b01382 .

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