具有近红外光控释功能的邻苯二酚改性壳聚糖基可注射生物黏合剂的制备及性能

王黎安; 葛志青; 汪谟贞; 葛学武

doi:10.11777/j.issn1000-3304.2020.20005

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具有近红外光控释功能的邻苯二酚改性壳聚糖基可注射生物黏合剂的制备及性能

论文 | 更新时间：2021-01-26

- 具有近红外光控释功能的邻苯二酚改性壳聚糖基可注射生物黏合剂的制备及性能
- Preparation and Properties of Catechol Modified Chitosan-based Injectable Biological Adhesives with Near-infrared-light-controlled Release Behavior
- 高分子学报 2020年51卷第12期页码：1335-1346
- 作者机构：
  
  中国科学院软物质化学重点实验室中国科学技术大学高分子科学与工程系　合肥 230026
- 作者简介：
  
  E-mail: pstwmz@ustc.edu.cn E-mail: pstwmz@ustc.edu.cn
  E-mail: xwge@ustc.edu.cn E-mail: xwge@ustc.edu.cn
- 基金信息：
  
  国家自然科学基金(基金号 51773189，51973205)和中央高校基本科研业务费专项资金(项目号 WK3450000004)资助
- DOI：10.11777/j.issn1000-3304.2020.20005
  中图分类号：
- 纸质出版日期：2020-10-21，
  
  网络出版日期：2020-8-12，
  
  收稿日期：2020-5-15，
  
  修回日期：2020-6-14，
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王黎安, 葛志青, 汪谟贞, 葛学武. 具有近红外光控释功能的邻苯二酚改性壳聚糖基可注射生物黏合剂的制备及性能[J]. 高分子学报, 2020,51(12):1335-1346.

Li-an Wang, Zhi-qing Ge, Mo-zhen Wang, Xue-wu Ge. Preparation and Properties of Catechol Modified Chitosan-based Injectable Biological Adhesives with Near-infrared-light-controlled Release Behavior[J]. Acta Polymerica Sinica, 2020,51(12):1335-1346.
王黎安, 葛志青, 汪谟贞, 葛学武. 具有近红外光控释功能的邻苯二酚改性壳聚糖基可注射生物黏合剂的制备及性能[J]. 高分子学报, 2020,51(12):1335-1346. DOI： 10.11777/j.issn1000-3304.2020.20005.

Li-an Wang, Zhi-qing Ge, Mo-zhen Wang, Xue-wu Ge. Preparation and Properties of Catechol Modified Chitosan-based Injectable Biological Adhesives with Near-infrared-light-controlled Release Behavior[J]. Acta Polymerica Sinica, 2020,51(12):1335-1346. DOI： 10.11777/j.issn1000-3304.2020.20005.

摘要

在制备邻苯二酚基团改性壳聚糖(CS-

C)的基础上，将CS-

C乙酸水溶液与

-甘油磷酸钠(

-GP)水溶液混合形成可注射溶胶，该溶胶加热至体温附近(37 °C)时发生溶胶-凝胶转化，得到无细胞毒性且有优良抗菌和黏附性能的水凝胶(CS-

-GP). 该水凝胶黏附在猪皮上的临界剥离强度可达4.9 kPa，是纯壳聚糖(CS)基水凝胶临界剥离强度(0.6 kPa)的8倍. 进一步在CS-

-GP溶胶形成过程中加入水溶性药物盐酸多西环素(DH)和近红外光热剂金纳米棒(AuNR)，即可以形成具有近红外光控释功能的CS-

-GP/DH/AuNR水凝胶，其负载的DH在808 nm近红外激光照射下的释放速率是无光照下的6倍. 本工作为开发高效可注射型多功能邻苯二酚改性壳聚糖基生物黏合剂提供了新思路.

Abstract

Chitosan (CS) hydrogel based adhesives have good biocompatibility

but poor adhesive property. In this paper

catechol modified chitosan (CS-

C) was synthesized by modifying CS with 3

4- dihydroxybenzoic acid. The mass ratio of catechol group of CS-

C is 14.4%. When the CS-

C acetic acid aqueous solution and the aqueous solution of

-glycerol phosphate disodium salt (

-GP) is mixed

an injectable CS-

-GP sol is formed

which can be transformed into CS-

-GP hydrogel under heating. The sol-gel transition time decreases exponentially with the increase of the temperature

and is around seven minutes at 37 °C. The CS-

-GP hydrogel has good biocompatibility

showing no cytotoxicity to Hela cells

MCF-7 cell

as well as 293T cells. It has excellent antibacterial properties on killing

Escherichia coli

and

Staphylococcus aureus

. It is noted that the CS-

-GP hydrogel has brilliant adhesion property. The CS-

-GP hydrogel can adhere to the porcine skin tightly. The critical peel strength is up to 4.9 kPa

eight times higher than that of the hydrogel prepared from unmodified CS (0.6 kPa). Furthermore

the water-soluble drug doxycycline hydrochloride (DH) and near-infrared (NIR) photothermal agent gold nanorods (AuNR) can be loaded into the CS-

-GP sol to form an injectable CS-

-GP/DH/AuNR sol

which can also be transformed into CS-

-GP/DH/AuNR hydrogel rapidly at 37 °C. The strong NIR photothermal conversion effect of the loaded AuNR offers the hydrogel a remarkable NIR light controlled release function. The release rate of the loaded DH will increase six times under the irradiation of 808 nm NIR laser

which indicates that the CS-

-GP/DH/AuNR hydrogel adhesive can also achieve a controlled wound healing. This work provides a new way for the development of safe

high-performance

and multifunctional injectable catechol modified chitosan based biological adhesives.

关键词

壳聚糖水凝胶可注射溶胶生物黏合剂近红外光控释

Keywords

Chitosan hydrogelInjectable solBiological adhesivesNear-infared-light-controlled release

references

Wang Yanhong(王艳红), Gu Hanqing(顾汉卿). Chinese Journal of Dialysis and Artificial Organs(透析与人工器官) , 2008 . 19 ( 3 ): 23 - 32.

Shi C M, Zhu Y, Ran X Z, Wang M, Su Y P, Cheng T M. J Surg Res , 2006 . 133 ( 2 ): 185 - 192 . DOI:10.1016/j.jss.2005.12.013http://doi.org/10.1016/j.jss.2005.12.013 .

Gong C Y, Wu Q J, Wang Y J, Zhang D D, Luo F, Zhao X, Wei Y Q, Qian Z Y. Biomaterials , 2013 . 34 ( 27 ): 6377 - 6387 . DOI:10.1016/j.biomaterials.2013.05.005http://doi.org/10.1016/j.biomaterials.2013.05.005 .

Mohamed A, Xing M. Int J Burn Trauma , 2012 . 2 ( 1 ): 29 - 41.

Annabi N, Rana D, Sani E S, Lara R P, Gifford J L, Fares M M, Mithieux S M, Weiss A S. Biomaterials , 2017 . 139 229 - 243 . DOI:10.1016/j.biomaterials.2017.05.011http://doi.org/10.1016/j.biomaterials.2017.05.011 .

Sun Liping(孙立苹), Du Yumin(杜予民), Chen Lingyun(陈凌云), Huang Ronghua(黄荣华), Chen Xiao(陈效). Acta Polymerica Sinica(高分子学报) , 2004 . ( 2 ): 191 - 195 . DOI:10.3321/j.issn:1000-3304.2004.02.007http://doi.org/10.3321/j.issn:1000-3304.2004.02.007 .

Tang Liming(唐黎明), Dai Huo(戴或). Acta Polymerica Sinica(高分子学报) , 2003 . ( 2 ): 426 - 429.

Han L, Wang M H, Li P F, Gan D L, Yan L W, Xu J L, Wang K F, Fang L M, Chan C W, Zhang H P, Yuan H P, and Lu X. ACS Appl Mater Interfaces , 2018 . 10 ( 33 ): 28015 - 28026 . DOI:10.1021/acsami.8b05314http://doi.org/10.1021/acsami.8b05314 .

Bi Sixin(毕思信), Yang Ning(杨宁), Yang Miaokun(杨淼坤), He Xiaoling(贺晓凌), Zhang Qingsong(张青松),Chen Li(陈莉), Zhu Zhengyan(朱争艳), Gao Yingtang(高英堂), Du Zhi(杜智). Acta Polymerica Sinica(高分子学报) , 2013 . ( 7 ): 888 - 895.

Hamedi H, Moradi S, Hudson S M, Tonelli A E. Carbohyd Polym , 2018 . 199 445 - 460 . DOI:10.1016/j.carbpol.2018.06.114http://doi.org/10.1016/j.carbpol.2018.06.114 .

Xu J K, Strandman S, Zhu J X X, Barralet J, Cerruti M. Biomaterials , 2015 . 37 395 - 404 . DOI:10.1016/j.biomaterials.2014.10.024http://doi.org/10.1016/j.biomaterials.2014.10.024 .

Chenite A, Chaput C, Wang D, Combes C, Buschmann M D, Hoemann C D, Leroux J C, Atkinson B L, Binette F, Selmani A. Biomaterials , 2000 . 21 ( 21 ): 2155 - 2161 . DOI:10.1016/S0142-9612(00)00116-2http://doi.org/10.1016/S0142-9612(00)00116-2 .

Kim S, Nishimoto S K, Bumgardner J D, Haggard W O, Gaber M W, Yang Y Z. Biomaterials , 2010 . 31 ( 14 ): 4157 - 4166 . DOI:10.1016/j.biomaterials.2010.01.139http://doi.org/10.1016/j.biomaterials.2010.01.139 .

Ryu J H, Lee Y, Kong W H, Park T G, Lee H. Biomacromolecules , 2011 . 12 ( 7 ): 2653 - 2659 . DOI:10.1021/bm200464xhttp://doi.org/10.1021/bm200464x .

Wang Z K, Nie J Y, Qin W, Hu Q L, Tang B Z. Nat Commun , 2016 . 7 12033 DOI:10.1038/ncomms12033http://doi.org/10.1038/ncomms12033 .

Lee H, Scherer N F, Messersmith P B. PNAS , 2006 . 103 ( 35 ): 12999 - 13003 . DOI:10.1073/pnas.0605552103http://doi.org/10.1073/pnas.0605552103 .

Waite J H, Andersen N H, Jewhurst S, Sun C. J Adhesion , 2005 . 81 ( 3-4 ): 297 - 317 . DOI:10.1080/00218460590944602http://doi.org/10.1080/00218460590944602 .

Fan X W, Lin L J, Dalsin J L, Messersmith P B. J Am Chem Soc , 2005 . 127 ( 45 ): 15843 - 15847 . DOI:10.1021/ja0532638http://doi.org/10.1021/ja0532638 .

Bilic G, Brubaker C, Messersmith P B, Mallik A S, Quinn T M, Haller C, Done E, Gucciardo L, Zeisberger S M, Zimmermann R, Deprest J, Zisch A H. Am J Obstet Gynecol , 2010 . 202 ( 1 ): 85e1 - 85e9 . DOI:10.1016/j.ajog.2009.07.051http://doi.org/10.1016/j.ajog.2009.07.051 .

Samyn P, Rühe J, and Biesalski M. Langmuir , 2010 . 26 ( 11 ): 8573 - 8581 . DOI:10.1021/la904629ahttp://doi.org/10.1021/la904629a .

Fujimoto K, Yamamura K, Osada T, Hayashi T, Nabeshima T, Matsushita M, Nishikimi N, Sakurai T, Nimura Y. J Biomed Mater Res A , 1997 . 36 ( 4 ): 564 - 567 . DOI:10.1002/(SICI)1097-4636(19970915)36:4<564::AID-JBM16>3.0.CO;2-Ahttp://doi.org/10.1002/(SICI)1097-4636(19970915)36:4<564::AID-JBM16>3.0.CO;2-A .

Chattopadhyay D P, Inamdar M S. Int J Polym Sci , 2010 . 1010 939536 .

Ye X C, Zheng C, Chen J, Gao Y Z, Murray C B. Nano Lett , 2013 . 13 ( 2 ): 765 - 771 . DOI:10.1021/nl304478hhttp://doi.org/10.1021/nl304478h .

Jing X, Mi H Y, Napiwocki B N, Peng X F,Turng L S. Carbon , 2017 . 125 557 - 570 . DOI:10.1016/j.carbon.2017.09.071http://doi.org/10.1016/j.carbon.2017.09.071 .

Liang Y P, Zhao X, Hu T L, Chen B J, Yin Z H, Ma P X, Guo B L. Small , 2019 . 15 ( 12 ): 1900046 DOI:10.1002/smll.201900046http://doi.org/10.1002/smll.201900046 .

Deng A P, Kang X, Zhang J, Yang Y, Yang S L. Mat Sci Eng C , 2017 . 78 1147 - 1154 . DOI:10.1016/j.msec.2017.04.109http://doi.org/10.1016/j.msec.2017.04.109 .

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