拓扑蛋白质的设计与合成

杨婷婷; 达晓娣; 张文彬

doi:10.11777/j.issn1000-3304.2020.20065

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拓扑蛋白质的设计与合成

专论 | 更新时间：2021-01-26

- 拓扑蛋白质的设计与合成
- Design and Biosynthesis of Topological Proteins
- 高分子学报 2020年51卷第8期页码：804-816
- 作者机构：
  
  北京分子科学国家研究中心高分子化学与物理教育部重点实验室软物质科学与工程中心北京大学化学与分子工程学院　北京 100871
- 作者简介：
  
  [ "张文彬，男，1981年生，现任北京大学化学与分子工程学院特聘研究员，博士生导师. 2004年和2010年分别获北京大学学士和美国阿克伦大学博士学位，之后先后在阿克伦大学和加州理工做博士后研究. 2013年8月加入北京大学化学学院. 主要研究方向为结合合成高分子和生物高分子的独特基元和设计理念，发展具有不寻常结构和性质的精密结构大分子材料. 获日本化学会杰出讲座奖(2017年)、国家杰出青年科学基金(2019年)等荣誉和人才计划" ]
- 基金信息：
  
  国家自然科学基金(基金号21925102，21991132)和北京分子科学国家研究中心2019年度创新研究项目(项目号 BNLMS-CXXM-202006)资助
- DOI：10.11777/j.issn1000-3304.2020.20065
  中图分类号：
- 纸质出版日期：2020-8，
  
  网络出版日期：2020-6-5，
  
  收稿日期：2020-3-13，
  
  修回日期：2020-4-4，
扫描看全文
杨婷婷, 达晓娣, 张文彬. 拓扑蛋白质的设计与合成[J]. 高分子学报, 2020,51(8):804-816.

Ting-ting Yang, Xiao-Di Da, Wen-Bin Zhang. Design and Biosynthesis of Topological Proteins[J]. Acta Polymerica Sinica, 2020,51(8):804-816.
杨婷婷, 达晓娣, 张文彬. 拓扑蛋白质的设计与合成[J]. 高分子学报, 2020,51(8):804-816. DOI： 10.11777/j.issn1000-3304.2020.20065.

Ting-ting Yang, Xiao-Di Da, Wen-Bin Zhang. Design and Biosynthesis of Topological Proteins[J]. Acta Polymerica Sinica, 2020,51(8):804-816. DOI： 10.11777/j.issn1000-3304.2020.20065.

摘要

拓扑蛋白质是一类具有复杂拓扑结构的非线性蛋白质，为抗体工程、工程酶、生物材料等领域提供了崭新的研究对象，具有重要的基础科学意义和应用价值. 本专论从拓扑高分子合成的重大挑战出发，总结了天然拓扑蛋白质的合成策略，详细阐述了如何灵活应用组装和反应协同构建人工拓扑蛋白质，讨论了拓扑蛋白质的表征方法，并提出了该领域进一步发展所面临的挑战和机遇.

Abstract

Topological proteins are proteins possessing non-linear backbones and nontrivial chemical topology. Since nascent polypeptide chains are strictly linear as defined by the translational mechanism of the cellular machinery

synthesis of topological proteins remains a huge challenge. By folding into various three-dimensional shapes

proteins can gain certain control over the spatial relationship of secondary motifs

however

the diversity of the chemical topology of the backbone remains largely untapped. The discovery of natural topological proteins in the past decades have inspired the researchers to explore the design and synthesis of artificial topological proteins. Meanwhile

the progresses in supramolecular chemistry and topological polymer chemistry have brought in various strategies for the synthesis of topological molecules in general. Among them

the “assembly-reaction” synergy seems to be a generally applicable and powerful one in creating unconventional structures. With genetically encoded entangling protein motifs and genetically encoded peptide-protein reactive pairs

different topological proteins have been prepared

including cyclic proteins

star proteins

branched proteins

tadpole proteins and protein catenanes. While structures and functions are well preserved in most cases

they also exhibit considerable advantages in terms of thermal stability and resistance to chemical denaturation and proteolytic digestion. Herein

begin with the major challenges in the synthesis of topological polymers

we summarize the biosynthesis of topological proteins in nature and the recent efforts to design and construct artificial topological proteins in chemistry. We will discuss the strategies for their synthesis and characterization

as well as the potential functional benefits of topological proteins. Finally

we will present our perspective on the challenges and opportunities of this emerging field.

关键词

拓扑蛋白质工程打结蛋白套索索烃组装-反应协同

Keywords

TopologyProtein engineeringKnot proteinLassoCatenaneAssembly-reaction synergy

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