基于固-气-液界面下细菌纤维素的合成

郑瑞珠; 石志军; 杨光

doi:10.11777/j.issn1000-3304.2020.20110

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基于固-气-液界面下细菌纤维素的合成

论文 | 更新时间：2021-01-26

- 基于固-气-液界面下细菌纤维素的合成
- Bacterial Cellulose Synthesis at Solid-Gas-Liquid Interface
- 高分子学报 2020年51卷第8期页码：942-948
- 作者机构：
  
  华中科技大学生命科学与技术学院　武汉 430074
- 作者简介：
  
  E-mail: gyang-hust@hust.edu.cn E-mail: gyang-hust@hust.edu.cn
- 基金信息：
  
  国家重点研发计划“政府间国际科技创新合作/港澳台科技创新合作”重点专项(项目号 2018YFE0123700)和国家自然科学基金(基金号 51973076，21774039)资助项目
- DOI：10.11777/j.issn1000-3304.2020.20110
  中图分类号：
- 纸质出版日期：2020-8，
  
  网络出版日期：2020-7-15，
  
  收稿日期：2020-4-26，
  
  修回日期：2020-6-6，
扫描看全文
郑瑞珠, 石志军, 杨光. 基于固-气-液界面下细菌纤维素的合成[J]. 高分子学报, 2020,51(8):942-948.

Rui-zhu Zheng, Zhi-jun Shi, Guang Yang. Bacterial Cellulose Synthesis at Solid-Gas-Liquid Interface[J]. Acta Polymerica Sinica, 2020,51(8):942-948.
郑瑞珠, 石志军, 杨光. 基于固-气-液界面下细菌纤维素的合成[J]. 高分子学报, 2020,51(8):942-948. DOI： 10.11777/j.issn1000-3304.2020.20110.

Rui-zhu Zheng, Zhi-jun Shi, Guang Yang. Bacterial Cellulose Synthesis at Solid-Gas-Liquid Interface[J]. Acta Polymerica Sinica, 2020,51(8):942-948. DOI： 10.11777/j.issn1000-3304.2020.20110.

摘要

细菌纤维素(bacterial cellulose

BC)是一种由葡糖木醋杆菌在生长运动过程中合成的具有纳米尺寸的纤维素. BC因其良好的生物相容性和机械性能使其在生物医学方面得到广泛研究和应用. 针对葡糖木醋杆菌氧气需求较敏感的特点，制造了表面具有不同结构微柱的光刻硅片，并进一步在硅片表面蒸镀一层十七氟癸基三甲氧基硅烷，使硅片具有超疏水特性，通过喷壶将不同液滴大小的菌液喷洒在该硅片上，形成“固-气-液”三相界面，解决有序微模板与细菌培养液之间的氧气需求问题，并根据液滴大小的不同，获得了许多精细的微尺寸纤维结构. 进一步地，在纤维网上培养细胞，探索了其在细胞捕获方面的应用.

Abstract

Bacterial cellulose (BC) is a nano-size cellulose synthesized by

Gluconacetobacter xylinus

during its growth and movement. Due to its good biocompatibility and good mechanical property

BC has been widely studied and applied in biomedicine and tissue engineering.

Gluconacetobacter xylinus

is a kind of aerobic bacteria

which preferentially synthesizes a BC film at the gas-liquid interface. Aiming at the sensitive characteristics of oxygen demand of

Gluconacetobacter xylinus

we referred the method of “solid-gas-liquid” three-phase interface formed on the interface of superhydrophobic nanomaterials in aqueous solution. Photolithographic silicon substrate with different micropillar structure on the surface were manufactured

and further evaporated a layer of heptadecafluorodecyltrimethoxysilane on the surface of silicon substrate

which made the silicon substrate superhydrophobic. Different sizes of medium with bacterial droplet sprayed on the silicon substrate through a sprinkling can

which made medium droplets were limited contact with the top of micropillar on the photolithographic silicon substrate to form a "solid-gas-liquid" interface

to solve the oxygen demand problem between ordered microstructure template and bacterial culture medium. Therefore

many micro-size fibers obtained due to the different sizes of droplet

including BC nanowire

BC wall

BC ball and BC network. When bacterial medium droplet rolling on pillar-structured photolithographic (~ 5 μL) formed BC nanowire between tips of two micropillar

large droplet (10 – 20 μL) formed BC wall between two micropillars

over 30 μL droplet formed BC network on micropillars. Furthermore

large droplet (10 – 20 μL) formed BC balls instead of BC network on large space (20 μm) vertical pillar-structured photolithographic silicon substrates

micro droplet (~ 1 μL) formed single BC ball on small space (5 μm) vertical pillar-structured photolithographic silicon substrates. In addition

HaCAT cells could culture on the BC network that means BC network synthesize in this way could capture adherent cells. Finally

challenges and opportunities of this BC synthesis method and its BC products toward future applications were discussed.

关键词

细菌纤维素葡糖木醋杆菌固-气-液界面细胞捕获

Keywords

Bacterial celluloseGluconacetobacter xylinusSolid-gas-liquid interfaceCell capture

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