固相纳米孔对核酸组装的“免标记、均相”表征和应用探索

祝振童; 吴瑞萍; 李冰凌

doi:10.11777/j.issn1000-3304.2021.21165

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固相纳米孔对核酸组装的“免标记、均相”表征和应用探索

专论 | 更新时间：2024-10-08

- 固相纳米孔对核酸组装的“免标记、均相”表征和应用探索
- Characterization and Application Exploration of Solid-state Nanopores for Label-free and Homogeneous Analysis of Nucleic Acid Assembly
- 高分子学报 2022年53卷第1期页码：4-14
- 作者机构：
  
  中国科学院长春应用化学研究所电分析化学国家重点实验室长春 130022
- 作者简介：
  
  [ "李冰凌，女，1982年生. 2004年于东北师范大学获得理学学士学位；2010年于中国科学院长春应用化学研究所获得理学博士学位，导师为董绍俊研究员. 2010~2015年于美国得州大学奥斯汀分校细胞和分子生物研究所从事博士后研究，导师为Andrew D. Ellington教授. 2015年4月起任中国科学院长春应用化学研究所研究员，现任中国医药生物技术协会生物诊断技术分会第三届委员会常委和《分析化学》青年编委. 主要从事核酸分子工程、重大疾病和传染病便携化诊断和单分子测量方面的基础和应用研究." ]
- 基金信息：
  
  国家自然科学基金(基金号22074136;22004102)
- DOI：10.11777/j.issn1000-3304.2021.21165
  中图分类号：
- 纸质出版日期：2022-01-20，
  
  网络出版日期：2021-10-22，
  
  收稿日期：2021-06-02，
  
  修回日期：2021-08-05，
- 稿件说明：
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祝振童,吴瑞萍,李冰凌.固相纳米孔对核酸组装的“免标记、均相”表征和应用探索[J].高分子学报,2022,53(01):4-14.

Zhu Zhen-tong,Wu Rui-ping,Li Bing-ling.Characterization and Application Exploration of Solid-state Nanopores for Label-free and Homogeneous Analysis of Nucleic Acid Assembly[J].ACTA POLYMERICA SINICA,2022,53(01):4-14.
祝振童,吴瑞萍,李冰凌.固相纳米孔对核酸组装的“免标记、均相”表征和应用探索[J].高分子学报,2022,53(01):4-14. DOI： 10.11777/j.issn1000-3304.2021.21165.

Zhu Zhen-tong,Wu Rui-ping,Li Bing-ling.Characterization and Application Exploration of Solid-state Nanopores for Label-free and Homogeneous Analysis of Nucleic Acid Assembly[J].ACTA POLYMERICA SINICA,2022,53(01):4-14. DOI： 10.11777/j.issn1000-3304.2021.21165.

摘要

随着核酸自组装领域的飞速发展，除了作为遗传信息的载体外，核酸成为了一种具有高操作自由度和无限可能性的功能材料. 基于核酸自组装原理的DNA纳米技术凭借其强大的可编辑性已经广泛应用于生物传感、纳米材料工程、医学诊疗以及分子计算机等领域. 纳米孔作为一种新兴的单分子分析技术具有高分辨、高通量、免标记等特点，近年来在基因测序、分子物理化学性质分析等领域展示出了极大的应用潜力. 作为一种新型高分辨表征技术，纳米孔已经在DNA纳米技术研究中崭露头角，被用于原位追踪和分析核酸分子的自组装行为. 另一方面，DNA纳米技术也为纳米孔传感所面临的技术瓶颈提供了更多样化的解决思路，如借助功能核酸(Aptamer或DNAzyme)和无酶扩增核酸分子线路实现纳米孔对待测物的特异性增敏检测. 本专论旨在通过对近期纳米孔技术与核酸自组装的跨领域研究成果进行系统性回顾，总结并展望纳米孔传感领域内核酸自组装的研究进展，以期为单分子生物分析、信息检索、基因分型和临床诊断等领域提供新思路和新方法.

Abstract

With the rapid development of the field of nucleic acid self-assembly

DNA becomes a functional and programmable material with high degree of free operation and unlimited possibilities. DNA nanotechnology based on various assembly principles has been widely used in biosensing

nanomaterial engineering

medical diagnosis and molecular computers. Nanopore analysis is an emerging

very effective single molecular technique for gene sequencing

molecular identification

and physical/chemical property characterization. Recently

due to the unique advantages such as high resolution

high throughput and label-free signalling

nanopore also raises increasing attention in the research of DNA nanotechnology

and has been explored as a new analytical method for various nucleic acid recognition and self-assembly behaviors. Meanwhile

DNA nanotechnology also provides more diversified solutions to the technical bottleneck of nanopore sensing

such as using functional nucleic acids (Aptamer or DNAzyme) and enzyme-free nucleic acid circuits to enhance detection sensitivity and specificity. This monograph systematically reviews these cross-field research progresses of nanopore technology and nucleic acid self-assemblies

which aims to summarize and prospect relevant new ideas and methods for single-molecule molecular analysis

information index

genotyping and clinical diagnosis.

关键词

固相纳米孔核酸纳米结构核酸分子线路免标记表征

Keywords

Soild-state nanoporeNucleic acid nanostructureNucleic acid circuitsLabel-free characterization

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