基于金属有机框架载体的免疫球蛋白G印迹材料的构筑及其识别性能研究

宋文琦; 杨雨璇; 刘文倩; 钱立伟

doi:10.11777/j.issn1000-3304.2021.21348

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基于金属有机框架载体的免疫球蛋白G印迹材料的构筑及其识别性能研究

研究论文 | 更新时间：2024-10-08

- 基于金属有机框架载体的免疫球蛋白G印迹材料的构筑及其识别性能研究
- Study on the Preparation and Recognition Performance of Immunoglobulin G Imprinted Material Based on Metal Organic Framework
- 高分子学报 2022年53卷第6期页码：653-662
- 作者机构：
  
  1.西京学院理学院西安 710123
  2.陕西科技大学轻工科学与工程学院西安 710021
  3.西北工业大学化学与化工学院西安 710072
- 作者简介：
  
  E-mail: qianliwei@mail.nwpu.edu.cn
- 基金信息：
  
  国家自然科学基金(基金号 21805177);陕西省重点研发计划项目(2021GY-240)
- DOI：10.11777/j.issn1000-3304.2021.21348
  中图分类号：
- 纸质出版日期：2022-06-20，
  
  网络出版日期：2022-04-22，
  
  收稿日期：2021-11-15，
  
  录用日期：2022-02-08
- 稿件说明：
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宋文琦,杨雨璇,刘文倩等.基于金属有机框架载体的免疫球蛋白G印迹材料的构筑及其识别性能研究[J].高分子学报,2022,53(06):653-662.

Song Wen-qi,Yang Yu-xuan,Liu Wen-qian,et al.Study on the Preparation and Recognition Performance of Immunoglobulin G Imprinted Material Based on Metal Organic Framework[J].ACTA POLYMERICA SINICA,2022,53(06):653-662.
宋文琦,杨雨璇,刘文倩等.基于金属有机框架载体的免疫球蛋白G印迹材料的构筑及其识别性能研究[J].高分子学报,2022,53(06):653-662. DOI： 10.11777/j.issn1000-3304.2021.21348.

Song Wen-qi,Yang Yu-xuan,Liu Wen-qian,et al.Study on the Preparation and Recognition Performance of Immunoglobulin G Imprinted Material Based on Metal Organic Framework[J].ACTA POLYMERICA SINICA,2022,53(06):653-662. DOI： 10.11777/j.issn1000-3304.2021.21348.

摘要

以人免疫球蛋白G (Ig G)作为模板分子，利用模板固定化与表面印迹技术相结合策略制备新型、高效的蛋白印迹材料. 为了增加印迹材料的分离效果，金属有机框架UIO-66被作为模板蛋白固定化的纳米载体. 同时为了提高印迹材料的识别性，在其制备中加入了两性离子单体. 研究结果表明，UIO-66晶体呈现出规则的正八面体形状，由它所制备的印迹材料(UIO-66@MIPs)展现出优异的比表面积(362.5 m

/g). 此外，通过对印迹聚合物制备过程中两性离子单体用量的优化，发现其占功能单体总质量20%时，可以获得最佳的识别性. 吸附实验研究结果表明，UIO-66@MIPs可在浓度为2.0 mg/mL的Ig G溶液中达到饱和吸附，其饱和吸附量可达217.4 mg/g，最大印迹因子为2.48，吸附平衡时间为50 min. 另外，选择性和竞争性吸附实验表明，与非印迹材料相比，UIO-66@MIPs对Ig G具有更强的亲合能力，并可以从混合蛋白溶液中高效分离Ig G. 本文的研究结果将为构筑高性能Ig G识别材料提供新的思路，对设计高效、廉价的新型冠状病毒检测试剂奠定理论基础.

Abstract

In this work

human immunoglobulin G (Ig G) is selected as the template

and the strategy of combining template immobilization and surface imprinting technology is used to prepare a new and efficient protein imprinting material (MIPs). In order to increase the separation effect of MIPs

the metal organic framework (UIO-66) is fabricated and used as a carrier for protein immobilization. Meanwhile

in order to improve the recognition of imprinting materials

zwitterionic monomers participate in the preparation of MIPs to improve their recognition ability. The research results showed that the UIO-66 crystal presented a regular octahedral shape

and the as-prepared MIPs exhibited an excellent specific surface area (362.5 m

/g). In addition

by optimizing the amount of zwitterionic monomer

it was found that the best recognition could be obtained when it accounted for 20% of the total mass of the functional monomer. The adsorption experiments also demonstrated that UIO-66@MIPs could achieve saturated adsorption in an Ig G solution with a concentration of 2.0 mg/mL

and its saturated adsorption capacity reached 217.4 mg/g with the imprinting factor of 2.48. Furthermore

the adsorption equilibrium time of UIO-66@MIPs was about 50 min. Moreover

the specific recognition experiment of UIO-66@MIPs showed that compared with non-imprinted materials

it could separate Ig G more effectively from the protein mixture solution

proving their excellent specific recognition capability.

关键词

蛋白质印迹技术金属有机框架两性离子单体

Keywords

Protein imprinting technologyMetal-organic frameworkZwitterionic monomer

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