唾液酸切除动力学对糖肽纤维形貌演变的影响

曾雅; 刘荣营; 李龙; 杜思南; 陈国颂

doi:10.11777/j.issn1000-3304.2022.22129

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唾液酸切除动力学对糖肽纤维形貌演变的影响

研究论文 | 更新时间：2024-10-08

- 唾液酸切除动力学对糖肽纤维形貌演变的影响
  增强出版
- Effects of Kinetics of Sialic Acid-removal on the Evolution of Glycopeptide Fibril Morphology
- 高分子学报 2022年53卷第12期页码：1466-1474
- 作者机构：
  
  聚合物分子工程国家重点实验室复旦大学高分子科学系上海 200438
- 作者简介：
  
  E-mail: 16110440025@fudan.edu.cn;
  guosong@fudan.edu.cn
- 基金信息：
  
  国家自然科学基金(基金号 ┣52125303);91956127┫
- DOI：10.11777/j.issn1000-3304.2022.22129
  中图分类号：
- 纸质出版日期：2022-12-20，
  
  网络出版日期：2022-09-09，
  
  收稿日期：2022-04-18，
  
  录用日期：2022-05-23
- 稿件说明：
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曾雅,刘荣营,李龙等.唾液酸切除动力学对糖肽纤维形貌演变的影响[J].高分子学报,2022,53(12):1466-1474.

Zeng Ya,Liu Rong-ying,Li Long,et al.Effects of Kinetics of Sialic Acid-removal on the Evolution of Glycopeptide Fibril Morphology[J].ACTA POLYMERICA SINICA,2022,53(12):1466-1474.
曾雅,刘荣营,李龙等.唾液酸切除动力学对糖肽纤维形貌演变的影响[J].高分子学报,2022,53(12):1466-1474. DOI： 10.11777/j.issn1000-3304.2022.22129.

Zeng Ya,Liu Rong-ying,Li Long,et al.Effects of Kinetics of Sialic Acid-removal on the Evolution of Glycopeptide Fibril Morphology[J].ACTA POLYMERICA SINICA,2022,53(12):1466-1474. DOI： 10.11777/j.issn1000-3304.2022.22129.

摘要

唾液酸酶介导的脱唾液酸过程在细胞表面唾液酸的动态调节中起着重要作用，并参与多种生理和病理过程. 对于这一重要过程的体外模拟和深入研究一方面将有利于深入理解许多唾液酸相关的生理过程，另一方面也为构建酶参与的仿生材料打下基础. 本文中我们利用表面高“表达”唾液酸的糖肽纳米纤维作为起始结构，然后通过在溶液中加入唾液酸酶来重现唾液酸酶介导的脱唾液酸过程. 结果显示加入的唾液酸酶确实导致了纤维表面唾液酸的切除并引发了糖肽纤维的形貌转变. 还通过改变唾液酸酶浓度来调控唾液酸切除动力学，结果显示唾液酸的切除速率决定了糖肽纤维的形貌演化路径，低的唾液酸切除速率导致初始的双螺旋糖肽纤维逐步演化为胶束，而高的唾液酸切除速率则使得初始的双螺旋糖肽纤维最终演化为扭曲的纳米带. 此外，也证实了这一策略在细胞培养方面的应用.

Abstract

The sialidase-mediated desia

lylation plays an important role in the dynamic regulation of sialic acid (SA) on the cell surface and is involved in a variety of physiological and pathological processes. For one thing

the

in vitro

simulation and in-depth study of this important process will help us deeply understand many physiological processes related to SA

and for another

it will also lay a foundation for the construction of biomimetic materials involving enzymes. Here

we used glycopeptide nanofibers with a large exposure of SA on the surface as the starting structure and then replicated the sialidase-mediated desialylation process by adding sialidase to the solution. The results showed that the added sialidase indeed led to the cleavage of SA on the fiber surface and thus triggered the morphological transformation of glycopeptide fibers. We also regulated the kinetics of SA removal by changing the sialidase concentration

and the results showed that the rate of removal of sialic acid determined the evolution path of glycopeptide fibers. Slow removal of SA led to the gradual evolution of the initial double-helix glycopeptide fibers into micelles

and the high rate of SA excision allowed the initial double-helical glycopeptide fibers to eventually evolve into twisted nanoribbons. In addition

the application of this strategy in cell culture has also been demonstrated.

关键词

糖肽自组装唾液酸酶形貌转变

Keywords

GlycopeptidesSelf-assemblySialidaseMorphology transformation

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