DNA超分子水凝胶的构筑、功能化与生物医学应用

潘玙璠; 丁雨樵; 董原辰; 刘冬生

doi:10.11777/j.issn1000-3304.2022.22380

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DNA超分子水凝胶的构筑、功能化与生物医学应用

专论 | 更新时间：2023-06-21

- DNA超分子水凝胶的构筑、功能化与生物医学应用
- Construction, Functionalization and Biomedical Application of DNA Supramolecular Hydrogels
- 高分子学报 2023年54卷第7期页码：1012-1027
- 作者机构：
  
  1.稀土新材料教育部工程研究中心清华大学化学系北京 100084
  2.中国人民大学化学系北京 100872
  3.中国科学院化学研究所中国科学院胶体、界面与化学热力学重点实验室北京 100190
  4.中国科学院大学北京 100149
- 作者简介：
  
  [ "董原辰，男，1986年生. 2010年于吉林大学生命科学学院获得学士学位；2015年于清华大学获得博士学位；2015~2018年，美国哈佛大学医学院从事博士后研究. 2018年回国后加入中国科学院化学研究所，现任研究员. 主要研究方向：框架诱导组装构建形貌尺寸可控的双亲组装体；DNA水凝胶及其生物应用；DNA双亲分子构建与组装的研究；基于DNA自组装的蛋白结构与功能研究；冷冻电镜在结构生物学中的应用." ]
  [ "刘冬生，男，1970年生. 1993年于中国科学技术大学高分子系获得学士学位；1999年于中国科学院化学研究所获得硕士学位，2002年于香港理工大学获得博士学位；2002~2003年，2003~2005年分别在香港理工大学与剑桥大学化学系、工程系及多学科研究纳米中心从事博士后研究. 2005年回国后加入国家纳米科学中心担任研究员，2009年加入清华大学化学系，现任教授、高分子物理与化学研究所所长. 2007年获得国家杰出青年基金资助，2011年成为英国皇家化学会会士，2013年担任科技部重大基础研究专项首席科学家，2015年成为教育部长江特聘教授，2016年入选中组部领军人才，2019年成为基金委创新研究群体学术带头人，同年入选中国化学会首批会士. 目前担任中国化学会高分子委员会秘书长. 主要研究方向为核酸纳米结构组装与应用，包括：核酸分子机器的构建与运动机制研究；以核酸大分子组装为基础的智能材料设计、制备及其在生物医学领域的应用研究；核酸超分子结构组装的生物学功能研究." ]
- 基金信息：
  
  国家自然科学基金(基金号 ┣21821001;21890731);21971248,21890730┫
- DOI：10.11777/j.issn1000-3304.2022.22380
  中图分类号：
- 纸质出版日期：2023-07-20，
  
  网络出版日期：2023-01-18，
  
  收稿日期：2022-11-10，
  
  录用日期：2022-12-08
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潘玙璠,丁雨樵,董原辰等.DNA超分子水凝胶的构筑、功能化与生物医学应用[J].高分子学报,2023,54(07):1012-1027.

Pan Yu-fan,Ding Yu-qiao,Dong Yuan-chen,et al.Construction, Functionalization and Biomedical Application of DNA Supramolecular Hydrogels[J].ACTA POLYMERICA SINICA,2023,54(07):1012-1027.
潘玙璠,丁雨樵,董原辰等.DNA超分子水凝胶的构筑、功能化与生物医学应用[J].高分子学报,2023,54(07):1012-1027. DOI： 10.11777/j.issn1000-3304.2022.22380.

Pan Yu-fan,Ding Yu-qiao,Dong Yuan-chen,et al.Construction, Functionalization and Biomedical Application of DNA Supramolecular Hydrogels[J].ACTA POLYMERICA SINICA,2023,54(07):1012-1027. DOI： 10.11777/j.issn1000-3304.2022.22380.

摘要

水凝胶具有与细胞外基质相似的组成与结构，是药物递送、组织工程以及体外细胞培养等生物医学领域重要的一类材料. 实现组成明确、生物相容、生物降解并且可功能化的水凝胶材料的构筑是高分子领域发展的重要挑战. DNA作为一种天然的生物大分子，天然具有明确的化学组成、可设计的序列以及独特的结构刚性，是构筑超分子水凝胶的优良材料. 本文回顾了DNA超分子水凝胶领域近年来的发展，主要介绍了纯/杂化DNA超分子水凝胶的构筑，总结了基于双链互补配对或物理缠结的纯DNA超分子水凝胶的构筑方法，讨论了物理与化学掺杂在杂化DNA超分子水凝胶构筑中的重要影响；系统介绍了DNA超分子水凝胶卓越的通透性、可调的力学强度以及动态的性质等物理特性，提出了DNA的刚性与动态性决定水凝胶物理性质的分子机制；总结了DNA超分子水凝胶的功能化策略，重点介绍了序列设计、化学修饰以及网络复合等方法；介绍了DNA超分子水凝胶在细胞三维培养与打印、组织工程以及免疫治疗和药物递送等生物医学领域的应用；最后，讨论了DNA超分子水凝胶领域发展面临的一些挑战与前景，为其未来应用前景提供新思路.

Abstract

Due to the similarity to extracellular matrix

hydrogels have been widely used in biomedical field such as drug delivery

tissue engineering and

in vitro

cell culture. Deoxyribonucleic acid (DNA) is known for the clear chemical composition

designable sequence and unique structural rigidity

which makes DNA an ideal material for construction of hydrogels. Based on our research

this review summarizes recent developments in DNA supramolecular hydrogels. Firstly

the construction strategies including the pure and hybrid DNA supramolecular hydrogels are introduced. While the construction of pure DNA supramolecular hydrogels is mainly based on base pairing or physical entanglement

covalent or non-covalent doping is also demonstrated to play important roles in preparing the hybrid hydrogels. Then

the physical properties of the DNA supramolecular hydrogels are discussed

such as the excellent permeability

tunable mechanical strength and dynamic properties. Next

the functionalization strategies are also summarized

including sequence design

chemical modification and network interpenetration. Furthermore

the applications of DNA supramolecular hydrogels in biomedicine are introduced

focusing on three-dimensional cell culture and printing

tissue engineering

immunotherapy and drug delivery. Finally

some challenges and future perspectives in the development of DNA supramolecular hydrogels are discussed. It is expected that with the flourishing development

DNA supramolecular hydrogels will have more applications.

关键词

DNA超分子水凝胶功能化生物医学应用

Keywords

DNA supramolecular hydrogelFunctionalizationBiomedical application

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