促内皮化<italic style="font-style: italic">β</italic>-多肽聚合物修饰的聚氨酯表面抗菌功能研究

周睿毅; 佘蕴芮; 武月铭; 张君宇; 王靖; 刘润辉

doi:10.11777/j.issn1000-3304.2022.22406

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促内皮化β-多肽聚合物修饰的聚氨酯表面抗菌功能研究

研究论文 | 更新时间：2023-06-21

- 促内皮化β-多肽聚合物修饰的聚氨酯表面抗菌功能研究
- Study on Antibacterial Function of Polyurethane Surface Modified with Endothelializable β-Peptide Polymer
- 高分子学报 2023年54卷第7期页码：1055-1063
- 作者机构：
  
  1.华东理工大学材料科学与工程学院教育部医用生物材料工程研究中心上海 200237
  2.华东理工大学深圳研究院深圳 518063
- 作者简介：
  
  E-mail: rliu@ecust.edu.cn
- 基金信息：
  
  国家自然科学基金(基金号 ┣21861162010);52203162┫;上海市优秀青年学术带头人项目(20XD1421400);中国博士后科学基金(基金号 2021M701198);博士后创新人才支持计划(BX20220108);华东理工大学深圳研究院自由探索类基础研究项目(2021Szvup042)
- DOI：10.11777/j.issn1000-3304.2022.22406
  中图分类号：
- 纸质出版日期：2023-07-20，
  
  网络出版日期：2023-03-22，
  
  收稿日期：2022-11-25，
  
  录用日期：2023-01-20
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周睿毅,佘蕴芮,武月铭等.促内皮化β-多肽聚合物修饰的聚氨酯表面抗菌功能研究[J].高分子学报,2023,54(07):1055-1063.

Zhou Rui-yi,She Yun-rui,Wu Yue-ming,et al.Study on Antibacterial Function of Polyurethane Surface Modified with Endothelializable β-Peptide Polymer[J].ACTA POLYMERICA SINICA,2023,54(07):1055-1063.
周睿毅,佘蕴芮,武月铭等.促内皮化β-多肽聚合物修饰的聚氨酯表面抗菌功能研究[J].高分子学报,2023,54(07):1055-1063. DOI： 10.11777/j.issn1000-3304.2022.22406.

Zhou Rui-yi,She Yun-rui,Wu Yue-ming,et al.Study on Antibacterial Function of Polyurethane Surface Modified with Endothelializable β-Peptide Polymer[J].ACTA POLYMERICA SINICA,2023,54(07):1055-1063. DOI： 10.11777/j.issn1000-3304.2022.22406.

摘要

由于血管移植物在植入过程中容易引起内皮损伤及微生物感染的风险，迫切需要研究能够促进表面内皮化并抗微生物感染的生物材料来降低这些风险发生的概率. 本工作将近期发现的具有促内皮化功能的

-多肽聚合物修饰到人工血管常用材料热塑型聚氨酯(TPU)表面，并研究其抗菌功能. 研究表明，

-多肽聚合物修饰表面对耐药革兰氏阳性菌和阴性菌表现出广谱的高效抗菌活性. 此外，该功能化TPU表面仍具有优异的选择性促内皮细胞黏附的能力，可满足人工血管移植物抗感染及促内皮化的多功能需求.

Abstract

Vascular grafts are prone to cause risks of endothelial damage and microbial infection during implantation

so there is an urgent need to find multifunctional biomaterials with endothelializable and antibacterial functions to reduce these risks. Host defense peptides (HDPs) have broad-spectrum antimicrobial activity and are widely used to inhibit microbial infections. However

the application of these peptides is limited due to their low stability

expensive and time-consuming synthesis. Therefore

in order to meet the multifunctional requirements of vascular grafts and address these limitations

endothelializable

-peptide polymer NM

was used as HDPs mimics for antibacterial functional studies in this work.

-Peptide polymer NM

was grafted to the surface of thermoplastic polyurethane (TPU)

commonly used in artificial vascular grafts

via

a process of O

plasma activation followed by brominated modification. The effective functionalization of TPU surface was confirmed by ellipsometry

X-ray photoelectron spectroscopy and water contact angle characterization. Antibacterial studies showed that NM

-modified TPU surface exhibited broad-spectrum antibacterial activity against multi-drug resistant Gram-positive and Gram-negative bacteria

as well as efficient contact-killing ability. Antimicrobial mechanism study indicated that NM

-modified TPU surface killed bacteria by interacting with bacterial membrane. Meanwhile

the functionalized TPU surface displayed excellent blood compatibility. In addition

-peptide polymer NM

-modified TPU surface maintained superior endothelial-selective cell adhesion

which can effectively reduce the risk of microbial infection and endothelial damage of vascular grafts

implying great potential in the treatment of cardiovascular disease.

关键词

β-多肽聚合物宿主防御肽抗菌内皮化

Keywords

β-Peptide polymerHost defense peptideAntibacterialEndothelialization

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促内皮化β-多肽聚合物修饰的聚氨酯表面抗菌功能研究

Study on Antibacterial Function of Polyurethane Surface Modified with Endothelializable β-Peptide Polymer

DOI：10.11777/j.issn1000-3304.2022.22406

摘要

Abstract

关键词

Keywords

references