基于静电纺丝的类海绵体材料用于溶菌酶的吸附行为

陈静; 刘畅; 于冰; 丛海林

doi:10.11777/j.issn1000-3304.2023.23114

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基于静电纺丝的类海绵体材料用于溶菌酶的吸附行为

研究论文 | 更新时间：2023-09-25

- 基于静电纺丝的类海绵体材料用于溶菌酶的吸附行为
- Adsorption Behavior of Lysozyme by Sponge-like Materials Based on Electrospinning
- 高分子学报 2023年54卷第10期页码：1632-1640
- 作者机构：
  
  1.青岛大学化学化工学院青岛 266071
  2.山东理工大学材料科学与工程学院淄博 255000
- 作者简介：
  
  E-mail: yubing198@qdu.edu.cn;
  E-mail: conghailin@sdut.edu.cn
- 基金信息：
  
  国家自然科学基金(基金号 ┣22074072);22274083┫
- DOI：10.11777/j.issn1000-3304.2023.23114
  中图分类号：
- 纸质出版日期：2023-10-20，
  
  网络出版日期：2023-08-15，
  
  收稿日期：2023-05-23，
  
  录用日期：2023-06-28
扫描看全文
陈静,刘畅,于冰等.基于静电纺丝的类海绵体材料用于溶菌酶的吸附行为[J].高分子学报,2023,54(10):1632-1640.

Chen Jing,Liu Chang,Yu Bing,et al.Adsorption Behavior of Lysozyme by Sponge-like Materials Based on Electrospinning[J].ACTA POLYMERICA SINICA,2023,54(10):1632-1640.
陈静,刘畅,于冰等.基于静电纺丝的类海绵体材料用于溶菌酶的吸附行为[J].高分子学报,2023,54(10):1632-1640. DOI： 10.11777/j.issn1000-3304.2023.23114.

Chen Jing,Liu Chang,Yu Bing,et al.Adsorption Behavior of Lysozyme by Sponge-like Materials Based on Electrospinning[J].ACTA POLYMERICA SINICA,2023,54(10):1632-1640. DOI： 10.11777/j.issn1000-3304.2023.23114.

摘要

用SiO

纳米纤维作为三维结构刚性支撑框架，以聚乙烯醇(PVA)/丁烷四羧酸(BTCA)的酯化交联反应物为功能性有机黏合剂，制备了一种高度羧基化的海绵体整体吸附材料(PVA/BTCA多孔材料)，得到的PVA/BTCA材料具有多孔结构. 作为一种吸附材料，通过水下循环压缩性能测试证明PVA/BTCA材料在水下拥有良好的结构稳定性. PVA/BTCA多孔材料的高度羧酸化和高孔隙率为蛋白吸附提供了基础条件，可以选择性吸附带正电荷的蛋白. 以溶菌酶为主要模型蛋白，PVA/BTCA多孔材料在pH=7时对溶菌酶的最大吸附量为1342.3 mg/g，PVA/BTCA多孔材料对溶菌酶的吸附的等温吸附模型与Freundlich吸附模型接近一致. 优秀的机械稳定性和化学结构稳定性赋予PVA/BTCA多孔材料可重复使用性，这项工作有望为蛋白分离方向提供一种新的分离材料.

Abstract

A highly carboxylated sponge monolithic adsorbent material (PVA/BTCA porous material) was prepared using SiO

nanofibers as a three-dimensional structural rigid support framework and an esterified cross-linking reactant of poly(vinyl alcohol) (PVA)/butane tetracarboxylic acid (BTCA) as a functional organic binder. As a kind of adsorption material

it is proven that PVA/BTCA material has good structural stability under water by underwater cyclic compression test. The highly carboxylated and high porosity of the PVA/BTCA porous material provided the basis for protein adsorption

which could selectively adsorb positively charged proteins. Using lysozyme as the main model protein

the maximum adsorption of PVA/BTCA porous material for lysozyme at pH=7 was 1342.3 mg/g. The isothermal adsorption model of PVA/BTCA porous material for the adsorption of lysozyme is in close agreement with the Freundlich adsorption model. The excellent mechanical stability and chemical structural stability confer reusability to the PVA/BTCA porous material. This work is expected to provide a new separation material for protein separation.

关键词

溶菌酶三维材料吸附聚乙烯醇

Keywords

LysozymeThree-dimensional materialAdsorptionPoly(vinyl alcohol)

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