荧光共振能量转移法研究高分子的溶液剪切构象

张昊昱; 陈葳

doi:10.11777/j.issn1000-3304.2024.24114

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荧光共振能量转移法研究高分子的溶液剪切构象

研究论文 | 更新时间：2024-12-24

- 荧光共振能量转移法研究高分子的溶液剪切构象
- Conformational Changes of Polymer Chains in a Shear Field Studied by Fluorescence Resonance Energy Transfer
- 高分子学报 2024年55卷第11期页码：1529-1537
- 作者机构：
  
  1.南京大学高性能高分子材料与技术教育部重点实验室南京 210023
  2.苏州大学江苏省新型高分子功能材料工程研究中心苏州 215123
- 作者简介：
  
  E-mail: weichen@nju.edu.cn
- 基金信息：
  
  国家自然科学基金(基金号 21790342);江苏省新型高分子功能材料工程研究中心开放课题(基金号 SDGC2311)
- DOI：10.11777/j.issn1000-3304.2024.24114
  中图分类号：
- CSTR：32057.14.GFZXB.2024.7261
- 收稿日期：2024-05-17，
  
  录用日期：2024-06-19，
  
  网络出版日期：2024-08-29，
  
  纸质出版日期：2024-11-20
- 稿件说明：
移动端阅览
张昊昱, 陈葳. 荧光共振能量转移法研究高分子的溶液剪切构象. 高分子学报, 2024, 55(11), 1529-1537

Zhang, H. Y.; Chen, W. Conformational changes of polymer chains in a shear field studied by fluorescence resonance energy transfer. Acta Polymerica Sinica, 2024, 55(11), 1529-1537
张昊昱, 陈葳. 荧光共振能量转移法研究高分子的溶液剪切构象. 高分子学报, 2024, 55(11), 1529-1537 DOI： 10.11777/j.issn1000-3304.2024.24114. CSTR： 32057.14.GFZXB.2024.7261.

Zhang, H. Y.; Chen, W. Conformational changes of polymer chains in a shear field studied by fluorescence resonance energy transfer. Acta Polymerica Sinica, 2024, 55(11), 1529-1537 DOI： 10.11777/j.issn1000-3304.2024.24114. CSTR： 32057.14.GFZXB.2024.7261.

摘要

从分子层面原位检测剪切场中高分子的微观构象不仅对研究高分子链运动与变形分子机理至关重要，也是开发高分子先进加工工艺的重要环节. 然而由于高分子链的构象变化尺度处于0.1~10 nm区间，现有的表征手段缺乏足够的分辨率. 为解决这一问题，在检测光路上加装可提供不同剪切速率的荧光样品池，对荧光仪进行改造，改造后的荧光仪能够实现剪切场中样品荧光信号的原位采集. 将荧光供体和受体基团通过共价键接入同一根高分子链，根据荧光共振能量转移效率与荧光供体和受体基团间的距离正相关的特点，采用荧光共振能量转移(FRET)光谱方法，通过分析标记在同一根高分子链上的荧光供体和受体基团间的FRET效率随剪切速率的变化，实现对高分子的溶液剪切构象的原位表征. 首次从分子层面原位观察到高分子链在剪切作用下，在稀溶液和亚浓溶液中，分别表现出“整体带动局部”和“局部带动整体”的构象变化模式. 为实验上开展高分子链构象演变的原位研究提供了新的方法和思路.

Abstract

The

in situ

detection of the microconformation of polymers in a shear field from the molecular level not only plays an important role for demonstrating the molecular mechanism of polymer chains' dynamic behavior such as deformation and diffusion

but also serves as a crucial link in developing advanced polymer processing technology. However

due to the conformational change scale of polymer chains falling within the range from 0.1 nm to 10 nm

conventional characterization methods

such as dynamic light scattering and fluorescence microscopy techniques

are difficult to meet the requirements of spatial resolution needed for characterization. To address this problem

the fluorometer was modified by adding a Couette rheofluorescence cell on the detection optical path. Since the Couette fluorescence cell can apply different shear rates to the sample

the modified fluorometer is able to detect

in situ

fluorescence signals of samples in a shear field. In order to characterize the conformational changes of the polymer chain

fluorescent donor and acceptor groups were labeled to a single polymer chain through covalent bonds Based on the positive correlation between the fluorescence resonance energy transfer (FRET) efficiency and the distance between the fluorescent donor and acceptor groups

a FRET spectroscopy has been used to achieve

in situ

characterization of the solution shear conformation of polymer chains by analyzing the change of the FRET efficiency between fluorescent donor and acceptor groups labeled on the same polymer chain as a function of the shear rate. For the first time

it was observed

in situ

at the molecular level that polymer chains under shear showed the conformational change modes of "whole driven by local" in dilute solutions and "local driven by whole" in semi-dilute solutions

respectively. This study provides new methods and ideas for experimentally carrying out

in situ

studies on the conformational evolution of polymer chains

and has an advancing effect on the development of molecular rheology.

关键词

Keywords

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