聚多肽囊泡声动力抗菌平台的构建及协同抗菌效果研究

杨敏; 韦平; 刘文浩; 陈敬华

doi:10.11777/j.issn1000-3304.2025.25015

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聚多肽囊泡声动力抗菌平台的构建及协同抗菌效果研究

研究论文 | 更新时间：2025-07-08

- 聚多肽囊泡声动力抗菌平台的构建及协同抗菌效果研究
- Polypeptosomes-based Sonodynamic Platform for Synergistic Antibacterial Therapy
- 高分子学报 2025年56卷第7期页码：1170-1179
- 作者机构：
  
  江南大学生命科学与健康工程学院无锡 214122
- 作者简介：
  
  E-mail: weiping@jiangnan.edu.cn
  chenjinghua@jiangnan.edu.cn
- 基金信息：
  
  国家自然科学基金(基金号 22305102);江苏省自然科学基金(基金号 BK20221097);中央高校基本科研业务费专项资金(基金号 JUSRP123042)
- DOI：10.11777/j.issn1000-3304.2025.25015
  中图分类号：
- CSTR：32057.14.GFZXB.2025.7387
- 收稿日期：2025-01-09，
  
  录用日期：2025-03-14，
  
  网络出版日期：2025-05-20，
  
  纸质出版日期：2025-07-20
- 稿件说明：
移动端阅览
杨敏, 韦平, 刘文浩, 陈敬华. 聚多肽囊泡声动力抗菌平台的构建及协同抗菌效果研究. 高分子学报, 2025, 56(7), 1170-1179

Yang, M.; Wei, P.; Liu, W. H.; Chen, J. H. Polypeptosomes-based sonodynamic platform for synergistic antibacterial therapy. Acta Polymerica Sinica, 2025, 56(7), 1170-1179
杨敏, 韦平, 刘文浩, 陈敬华. 聚多肽囊泡声动力抗菌平台的构建及协同抗菌效果研究. 高分子学报, 2025, 56(7), 1170-1179 DOI： 10.11777/j.issn1000-3304.2025.25015. CSTR： 32057.14.GFZXB.2025.7387.

Yang, M.; Wei, P.; Liu, W. H.; Chen, J. H. Polypeptosomes-based sonodynamic platform for synergistic antibacterial therapy. Acta Polymerica Sinica, 2025, 56(7), 1170-1179 DOI： 10.11777/j.issn1000-3304.2025.25015. CSTR： 32057.14.GFZXB.2025.7387.

摘要

抗生素长期不合理的乱用、滥用，导致细菌耐药性不断增强，严重威胁国民健康. 因此，亟需寻找一种新型、不易诱发耐药性的细菌感染治疗方法，以应对后抗生素时代的挑战. 针对该问题，成功设计出一种基于聚己内酯(PCL)和聚谷氨酸(PGA)的两亲性嵌段聚合物PCL

-PGA

，并自组装形成具有中空结构和双层膜的聚合物囊泡. 利用聚合物囊泡独特的结构特性，在其亲水空腔包载广谱抗生素药物头孢曲松(CRO)，疏水双层膜同步负载声敏剂二氢卟吩(Ce6)，构建声动力疗法联合抗生素多功能抗菌平台. 体外抗菌实验结果表明，在超声作用下，同时负载Ce6和CRO的聚合物囊泡可产生大量具有抗菌活性的活性氧(ROS)，与抗生素CRO协同作用，对耐CRO的大肠杆菌菌株具有显著的抗菌效果，其菌落总数由4.69×10

CFU/mL降低至3.17×10

CFU/mL. 该声动力疗法联合抗生素多功能抗菌平台的构建实现了协同抗菌，有望为新型抗菌药物的研发奠定实验基础.

Abstract

Bacterial infections constitute a substantial proportion of human morbidity and mortality

posing significant threats to public health and imposing considerable socioeconomic burdens annually. Although traditional antibiotics have a positive effect on killing bacteria

their therapeutic efficiency is greatly reduced due to the emergence of antibiotic-resistant pathogens. Therefore

there is an urgent need to seek an alternative treatment approach against antibiotic-resistant bacterial infections. Recently

some new antibiotics-free and non-invasive antibacterial approaches have been developed

including photothermal therapy

photodynamic therapy and ultrasound-triggered sonodynamic therapy (SDT). Among these approaches

SDT is the most promising bacterial infection treatment modality due to its deep tissue penetration capability and effectiveness against antibiotic-resistant bacteria. In this work

we propose a combined sonodynamic-antibiotic therapy based on poly(

ε‍

-caprolactone)-block-poly(glutamic acid) polymer vesicle. The hydrophilic cavity and the hydrophobic membrane of polymer vesicles can function as different modules for the simultaneous delivery of a broad-spectrum antibiotic drug ceftriaxone (CRO) and a photosensitizer chlorin e6 (Ce6). These multifunctional nanoplatforms exhibited enhanced antibacterial activity and could efficiently generate reactive oxygen species (ROS) upon ultrasound activation.

In vitro

antibacterial results indicated that under ultrasound exposure

the polymer vesicles simultaneously loaded with Ce6 and CRO exhibited a remarkable antibacterial effect against CRO-tolerant

Escherichia coli

strains

reducing the total colony count from 4.69×10

CFU/mL to 3.17×10

CFU/mL. The construction of this multifunctional antibacterial platform combining sonodynamic therapy and antibiotics can achieve synergistic antibacterial effects and is expected to lay an experimental foundation for the rese

arch and development of new antibacterial drugs.

关键词

Keywords

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