可溶性透明质酸微针经皮递送迷迭香酸协同光热抗菌的研究

徐婷; 葛黎明; 徐志朗; 李德富; 穆畅道

doi:10.11777/j.issn1000-3304.2025.25043

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可溶性透明质酸微针经皮递送迷迭香酸协同光热抗菌的研究

研究论文 | 更新时间：2025-06-27

- 可溶性透明质酸微针经皮递送迷迭香酸协同光热抗菌的研究
- Dissolving Hyaluronic Acid-based Microneedles with Antibacterial Activity Achieved by Photothermal Antibacterial Effect in Coordination with Transdermal Delivery of Rosmarinic Acid
- 高分子学报 2025年页码：1-11
- 作者机构：
  
  四川大学化学工程学院制药与生物工程系成都 610065
- 作者简介：
  
  E-mail: cdmu@scu.edu.cn
- 基金信息：
  
  国家自然科学基金(基金号 22178230);四川省国际港澳台科技创新合作项目(基金号 2023YFH0088)
- DOI：10.11777/j.issn1000-3304.2025.25043
  中图分类号：
- CSTR：32057.14.GFZXB.2025.7434
- 收稿日期：2025-02-19，
  
  录用日期：2025-06-03，
  
  网络出版日期：2025-06-27，
- 稿件说明：
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徐婷, 葛黎明, 徐志朗, 李德富, 穆畅道. 可溶性透明质酸微针经皮递送迷迭香酸协同光热抗菌的研究. 高分子学报, doi: 10.11777/j.issn1000-3304.2025.25043

Xu, T.; Ge, L. M.; Xu, Z. L.; Li, D. F.; Mu, C. D. Dissolving hyaluronic acid-based microneedles with antibacterial activity achieved by photothermal antibacterial effect in coordination with transdermal delivery of rosmarinic acid. Acta Polymerica Sinica, doi: 10.11777/j.issn1000-3304.2025.25043
徐婷, 葛黎明, 徐志朗, 李德富, 穆畅道. 可溶性透明质酸微针经皮递送迷迭香酸协同光热抗菌的研究. 高分子学报, doi: 10.11777/j.issn1000-3304.2025.25043 DOI： CSTR： 32057.14.GFZXB.2025.7434.

Xu, T.; Ge, L. M.; Xu, Z. L.; Li, D. F.; Mu, C. D. Dissolving hyaluronic acid-based microneedles with antibacterial activity achieved by photothermal antibacterial effect in coordination with transdermal delivery of rosmarinic acid. Acta Polymerica Sinica, doi: 10.11777/j.issn1000-3304.2025.25043 DOI： CSTR： 32057.14.GFZXB.2025.7434.

摘要

抗菌可溶性微针能有效突破皮肤角质层屏障，实现无痛、微创、安全和高效的经皮给药，在浅表皮肤疾病和细菌生物膜感染的治疗中发挥着重要作用. 以低分子量和中分子量透明质酸混合物为可溶性微针基材，采用分层浇筑法在针尖部位负载光热转换剂聚多巴胺纳米颗粒(PDA NPs)，在针体部位负载抗菌活性药物迷迭香酸，制备了一种协同光热治疗和透皮给药的抗菌可溶性透明质酸微针(PDA-RA-HA MNs). PDA-RA-HA MNs微针贴片具有良好的柔韧性，针体为高约558 μm的四棱锥形. PDA-RA-HA MNs具有较高的机械强度(0.76 N/针)和韧性，可以刺破皮肤角质层直达真皮层. PDA-RA-HA MNs由于针尖负载PDA NPs而具有较强的光热性能，经近红外光(NIR)照射120 s即可升温至55 ℃以实现低温光热治疗，从而大大减少对正常皮肤组织造成的热损伤. 此外，PDA-RA-HA MNs在近红外光的照射下对痤疮丙酸杆菌(

P. acnes

)的抑菌率超过90%，展现出优异的抗菌活性. 因此，基于光热抗菌和递送抗菌药物的协同抗菌策略构建的可溶性透明质酸微针，在痤疮等浅表皮肤疾病的治疗中具有潜在的应用前景.

Abstract

Antibacterial dissolving microneedles can effectively break through the skin cuticle barrier and achieve painless

minimally invasive

safe and efficient transdermal drug delivery

which plays an important role in the treatment of superficial skin diseases and bacterial biofilm infections. Herein

the antibacterial dissolving hyaluronic ac

id-based microneedles (PDA-RA-HA MNs) were fabricated via the layered micromolding method. The combination of medium- and low-molecular-weight hyaluronic acids for the preparation of microneedles can make up for the deficiency of mechanical strength and toughness of single material. Moreover

the photothermal conversion agent polydopamine nanoparticle (PDA NPs) was loaded on the needle tip

while the antibacterial agent rosmarinic acid was loaded on the needle body to endow microneedles with good antibacterial activity. The prepared PDA-RA-HA MNs microneedles patch showed good flexibility

which can bend to fit the skin well. All the needles were in the shape of a square pyramid

with a height of approximately 558 μm. PDA-RA-HA MNs can withstand a loaded force of about 0.76 N/needle

and can maintain the structural integrity after mechanical compression

indicating good mechanical strength and toughness. PDA-RA-HA MN can penetrate the stratum corneum of the skin

directly reaching the dermis layer

and the micro channels were formed in the skin tissue after microneedles dissolving

thereby achieving efficient transdermal drug delivery. PDA-RA-HA MNs have strong photothermal properties due to the loaded PDA NPs on the needle tip. They can be heated up to 55 ℃ after 120 s of NIR irradiation to achieve low temperature photothermal treatment

thus greatly reducing the heat damage caused to normal skin tissue. It is worth noting that PDA-RA-HA MNs showed excellent antibacterial activity against

P. acnes

with over 90% of bacteria inhibition efficiency under NIR irradiation. As expected

the robust antibacterial activity of PDA-RA-HA MNs was achieved by the photothermal antibacterial effect of PDA NPs in coordination with the transdermal delivery of rosmarinic acid. Therefore

the fabricated antibacterial dissolving hyaluronic acid-based microneedles show potential application prospects in the efficient killing of deeply colonized pathogenic bacteria for the treatment of superficial skin diseases such a

s acne vulgaris.

关键词

Keywords

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