基于恒温光热水凝胶的噬菌体-抗生素-光热多模态协同抗菌治疗

张婧怡; 符豪; 吕树一; 肖铭慧; 刘博; 田亮; 李华龙; 郑沁洋; 朱春雷

doi:10.11777/j.issn1000-3304.2026.26046

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基于恒温光热水凝胶的噬菌体-抗生素-光热多模态协同抗菌治疗

研究论文 | 更新时间：2026-04-09

- 基于恒温光热水凝胶的噬菌体-抗生素-光热多模态协同抗菌治疗
- Thermostatic Photothermal Hydrogel-based Multimodal Synergistic Antibacterial Therapy Integrating Bacteriophages, Antibiotics, and Photothermal Treatment
- 高分子学报 2026年页码：1-17
- 作者机构：
  
  南开大学化学学院高分子化学研究所功能高分子材料教育部重点实验室天津 300071
- 作者简介：
  
  E-mail: chunlei.zhu@nankai.edu.cn
- 基金信息：
  
  中央高校基本科研业务费(63251169);天津市科技计划项目(23JCZDJC00860)
- DOI：10.11777/j.issn1000-3304.2026.26046
  中图分类号：
- CSTR：32057.14.GFZXB.2026.7577
- 收稿：2026-02-08，
  
  录用：2026-03-13，
  
  网络首发：2026-04-09，
- 稿件说明：
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张婧怡, 符豪, 吕树一, 肖铭慧, 刘博, 田亮, 李华龙, 郑沁洋, 朱春雷. 基于恒温光热水凝胶的噬菌体-抗生素-光热多模态协同抗菌治疗. 高分子学报, doi: 10.11777/j.issn1000-3304.2026.26046.

Zhang, J. Y.; Fu, H.; Lv, S. Y.; Xiao, M. H.; Liu, B.; Tian, L.; Li, H. L.; Zheng, Q. Y.; Zhu, C. L. Thermostatic photothermal hydrogel-based multimodal synergistic antibacterial therapy integrating bacteriophages, antibiotics, and photothermal treatment. Acta Polymerica Sinica (in Chinese), doi: 10.11777/j.issn1000-3304.2026.26046.
张婧怡, 符豪, 吕树一, 肖铭慧, 刘博, 田亮, 李华龙, 郑沁洋, 朱春雷. 基于恒温光热水凝胶的噬菌体-抗生素-光热多模态协同抗菌治疗. 高分子学报, doi: 10.11777/j.issn1000-3304.2026.26046. DOI： CSTR： 32057.14.GFZXB.2026.7577.

Zhang, J. Y.; Fu, H.; Lv, S. Y.; Xiao, M. H.; Liu, B.; Tian, L.; Li, H. L.; Zheng, Q. Y.; Zhu, C. L. Thermostatic photothermal hydrogel-based multimodal synergistic antibacterial therapy integrating bacteriophages, antibiotics, and photothermal treatment. Acta Polymerica Sinica (in Chinese), doi: 10.11777/j.issn1000-3304.2026.26046. DOI： CSTR： 32057.14.GFZXB.2026.7577.

摘要

细菌感染对全球公共卫生构成严峻挑战，亟需开发新型高效的抗菌治疗策略. 本研究构建了一种兼具热响应与恒温调控能力的双层水凝胶递送系统，用于实现噬菌体的按需大量释放，并与抗生素协同用于细菌感染治疗. 该系统通过在下层水凝胶中引入光热纳米材料，在激光照射下，光热材料产热并诱导水凝胶发生相变收缩，从而触发噬菌体可控释放；与此同时，上层水凝胶通过相变诱导的光散射效应限制激光穿透，使体系温度精准维持在水凝胶相变点以下，有效避免噬菌体失活和组织过热损伤. 该控释系统与抗生素联合应用可实现对野生型大肠杆菌99.9%的高效杀灭，并在细菌感染伤口模型中显著促进伤口愈合. 本研究构建了一种集按需控释、智能温控与协同抗菌于一体的多模态协同治疗平台，为细菌感染性伤口的修复提供了新策略.

Abstract

Bacterial infections pose a severe challenge to global public health

necessitating the development of novel and efficient antibacterial therapeutic strategies. In this study

a bilayer hydrogel delivery system with thermal responsiveness and thermostatic regulation was constructed to achieve on-demand burst release of bacteriophages and synergistic treatment in combination with antibiotics. By incorporating photothermal nanomaterials into the bottom hydrogel layer

laser irradiation induces localized heat generation

triggering a phase transition-driven contraction of the hydrogel and consequently enabling controlled phage release. Meanwhile

the upper hydrogel layer

devoid of photothermal components

restricted laser penetration through a phase transition-induced light-scattering effect

thereby precisely maintaining the system temperature below the hydrogel transition threshold and effectively preventing phage inactivation and tissue overheating.

In vitro

antibacterial assay demonstrated that the combined application of the controlled-release system and antibiotics achieves 99.9% eradication of wild-type

Escherichia coli

(WT

E. coli

). Furthermore

in a bacteria-infected wound model

this synerg

istic therapeutic strategy significantly accelerated wound healing. Overall

this work presents a multimodal synergistic therapeutic platform integrating on-demand release

intelligent thermal regulation

and synergistic antibacterial activity

providing a promising strategy for the treatment of bacteria-infected wounds.

关键词

Keywords

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