A photoaffinity labeling molecule

4-azidobenzoic acid

was synthesized and introduced into P(LA-co-DHP) to form a photoactive copolymer P(LA-co-DAP).Tests showed that proteins could be immobilized on the polymer fibers of P(LA-co-DAP) within 5 min through a photoaffinity reaction.This suggested potential applications of photoaffinity coupling in protein immobilization and protein detection.Furthermore

PEG and 4-azidobenzoic acid were reacted in solution under UV irradiation to achieve the modification on PEG chains.The PEG molecules assembled on gold surface were reacted also with 4-azidobenzoic acid under UV irradiation.BSA adsorption test by FT-SPR showed that the 4-azidobenzoic acid moieties were successfully attached to the PEG segments so that the protein adsorption ability of the PEG surface was changed.In this way

functional groups likePhCOOH were introduced onto PEG chains in a convenient and quick manner.This would offer a new method to achieve the modification of the polymers lacking of functional groups.