半导体聚合物纳米点生物荧光成像探针进展

杨宏伟; 范庆瑞; 王健君

doi:10.11777/j.issn1000-3304.2024.24214

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半导体聚合物纳米点生物荧光成像探针进展

综述 | 更新时间：2025-02-27

- 半导体聚合物纳米点生物荧光成像探针进展
- Recent Advances of Semiconductor Polymer Nanodots for Fluorescence Bioimaging
- 高分子学报 2025年56卷第3期页码：396-407
- 作者机构：
  
  中国科学院理化技术研究所北京 100190
- 作者简介：
  
  [ "王健君，男，1976年生. 中国科学院理化技术研究所研究员，博士生导师. 1999、2002年，于华东理工大学分别获得学士、硕士学位；2006年获德国美因茨大学博士学位；2007年5月担任德国马普高分子所课题组长；2010年2月担任中国科学院化学研究所研究员/课题组长；2023年5月在中国科学院理化技术研究所工作，任研究员/课题组组长. 主要研究方向为防冰高分子材料设计、制备与应用、冰晶形成分子机制研究、控冰新材料的创制并应用于细胞组织和器官的低温保存等领域." ]
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2024.24214
  中图分类号：
- CSTR：32057.14.GFZXB.2024.7304
- 收稿日期：2024-08-16，
  
  录用日期：2024-10-08，
  
  网络出版日期：2024-12-17，
  
  纸质出版日期：2025-03-20
- 稿件说明：
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杨宏伟, 范庆瑞, 王健君. 半导体聚合物纳米点生物荧光成像探针进展. 高分子学报, 2025, 56(3), 396-407

Yang, H. W,; Fan, Q. R.; Wang, J. J. Recent advances of semiconductor polymer nanodots for fluorescence bioimaging. Acta Polymerica Sinica, 2025, 56(3), 396-407
杨宏伟, 范庆瑞, 王健君. 半导体聚合物纳米点生物荧光成像探针进展. 高分子学报, 2025, 56(3), 396-407 DOI： 10.11777/j.issn1000-3304.2024.24214. CSTR： 32057.14.GFZXB.2024.7304.

Yang, H. W,; Fan, Q. R.; Wang, J. J. Recent advances of semiconductor polymer nanodots for fluorescence bioimaging. Acta Polymerica Sinica, 2025, 56(3), 396-407 DOI： 10.11777/j.issn1000-3304.2024.24214. CSTR： 32057.14.GFZXB.2024.7304.

摘要

荧光成像技术因其非侵入性、高时空分辨率和高灵敏度，在生命科学研究领域中备受关注. 作为荧光成像技术的基础工具，发展高性能的荧光探针成为提高成像质量的关键途径之一. 随着纳米材料科学领域的迅速发展，荧光纳米颗粒探针因其克服了传统有机染料和荧光蛋白在荧光效率和光稳定性方面的不足，逐渐成为荧光成像领域的有力竞争者. 近年来，半导体聚合物纳米点(Pdots)因其粒径小、亮度高、稳定性强等特性在荧光纳米探针领域中备受瞩目. 本文首先回顾了Pdots的发展历程和制备策略，并总结了Pdots作为荧光纳米探针在光物理特性上所具备的优势. 此外重点介绍了Pdots作为一类性能优异的荧光纳米探针在荧光标记成像、超分辨荧光成像和活体生物成像中的最新应用进展. 最后，分析了当前Pdots作为荧光探针存在的一些优点和局限性，并探讨了该类纳米探针在未来存在的主要发展方向和应用前景，为Pdots在生命科学成像领域中的更广泛应用开辟新的可能性.

Abstract

Fluorescence imaging technology has garnered significant attention in the field of life sciences due to its non-invasive nature

high spatial and temporal resolution

and high sensitivity. As a fundamental tool in fluorescence imaging technology

the development of high-performance fluorescent probes is one of the key approaches to improving imaging quality. With the rapid development of nanomaterial science

fluorescent nanoparticle probes have emerged as formidable contenders in fluorescence imaging

overcoming the deficiencies of traditional organic dyes and fluorescent proteins in terms of fluorescence efficiency and photostability. In recent years

semiconductor polymer nanodots (Pdots) have attracted considerable attention in the field of fluorescent nanoprobes due to their small particle size

high brightness

and great photostability. In this review

we first summarized the development history and preparation strategies of Pdots

and organized the advantages of Pdots as fluorescent nanoprobes in terms of photophysical properties. In addition

this review focuses on the latest applications of Pdots as a class of high-performance fluorescent nanoprobes in fluorescence labeling imaging

super-resolution fluorescence imaging

and

in vivo

biological imaging. Finally

we analyzed the advantages and limitations of Pdots as fluorescent probes

and explored the main development directions and application prospects of this nanoprobe in the future

which creates new opportunities for broader applications of Pdots in life science imaging.

关键词

Keywords

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