高分子材料在蛋白分离应用中的研究进展

孙颖; 王伟杰; 姚焰; 刘青青; 陈鱼; 毛晓卉; 朱丽萍; 朱美芳

doi:10.11777/j.issn1000-3304.2025.24267

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高分子材料在蛋白分离应用中的研究进展

综述 | 更新时间：2025-04-27

- 高分子材料在蛋白分离应用中的研究进展
- 暂无标题
- 高分子学报 2025年页码：1-20
- 作者机构：
  
  1.东华大学先进纤维材料全国重点实验室材料科学与工程学院上海 201620
  2.康复大学康复科学与工程学院青岛 266114
- 作者简介：
  
  [ "毛晓卉，女，1990年生. 2012年毕业于南开大学化学学院分子科学与工程系，获南开大学理学学士学位和天津大学工学学士学位. 2015、2021年分别获加拿大阿尔伯塔大学硕士和博士学位. 2023年入职东华大学材料科学与工程学院. 获上海市高层次人才计划(海外)，主持国家自然科学青年基金. 近年来，在专业领域相关期刊发表论文30余篇. 研究方向为基于原子力显微镜探测分子间相互作用，主要应用领域有活性分子在油-水界面的组装、乳液稳定性调控、黏结剂分子设计." ]
  [ "朱丽萍，女，1981年生. 东华大学获学士和硕士学位，美国科罗拉多矿业学院获材料学博士学位，东华大学材料科学与工程学院副研究员、博士生导师，国家高层次青年人才，联合国环境署臭氧环境影响评估委员会委员. 2019年至今以第一或通讯作者身份在Acc. Mater. Res.、Matter、Carbohyd. Polym.、J. Colloid Interface Sci.等期刊发表论文25篇，主持国家自然科学基金面上项目1项、企业委托项目3项，承担科技部国家重点研发计划课题任务1项、国家自然科学基金联合基金项目课题1项. 获中国纺织联合会科技进步奖一等奖(3/15)、中国材料研究学会科学技术一等奖(6/15). 主要从事生物基医用高分子材料及纤维膜材料改性制备、材料表面改性及表界面性能研究." ]
- 基金信息：
  
  国家自然科学基金委面上项目(基金号52373098);国家重点研发计划(2021YFA1201300);福建省清源创新实验室重大专项(00122006);中央高校基本科研业务费专项资金(基金号 ┣2232024Y-01);2232024D-05┫
- DOI：10.11777/j.issn1000-3304.2025.24267
  中图分类号：
- CSTR：32057.14.GFZXB.2025.7377
- 收稿日期：2024-11-14，
  
  录用日期：2025-03-06，
  
  网络出版日期：2025-04-27，
- 稿件说明：
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孙颖, 王伟杰, 姚焰, 刘青青, 陈鱼, 毛晓卉, 朱丽萍, 朱美芳. 高分子材料在蛋白分离应用中的研究进展. 高分子学报, doi: 10.11777/j.issn1000-3304.2025.24267

Sun, Y.; Wang, W. J.; Yao, Y.; Liu, Q. Q.; Chen, Y.; Mao, X. H.; Zhu, L. P.; Zhu, M. F. Advances in polymer materials for protein separation applications. Acta Polymerica Sinica, doi: 10.11777/j.‍issn1000-3304.2025.24267
孙颖, 王伟杰, 姚焰, 刘青青, 陈鱼, 毛晓卉, 朱丽萍, 朱美芳. 高分子材料在蛋白分离应用中的研究进展. 高分子学报, doi: 10.11777/j.issn1000-3304.2025.24267 DOI： CSTR： 32057.14.GFZXB.2025.7377.

Sun, Y.; Wang, W. J.; Yao, Y.; Liu, Q. Q.; Chen, Y.; Mao, X. H.; Zhu, L. P.; Zhu, M. F. Advances in polymer materials for protein separation applications. Acta Polymerica Sinica, doi: 10.11777/j.‍issn1000-3304.2025.24267 DOI： 10.11777/j.issn1000-3304.2025.24267. CSTR： 32057.14.GFZXB.2025.7377.

摘要

蛋白质是构成生命组织、调节生理功能、为生命活动提供能量等所必需的重要物质. 随着蛋白质组学技术的提出及生物技术的迅速发展，对蛋白质高效分离的需求日益增长. 高分子材料凭借其良好的稳定性、智能响应性、可设计性及易加工性等特点成为蛋白分离领域的重要材料. 本文依据蛋白质自身物理特性，基于高分子官能团与蛋白的相互作用机理，将高分子分为中性、正电、负电以及可调节电荷体系，综述了其在蛋白分离技术中的应用，并展望了高分子材料在蛋白分离领域的未来发展.

Abstract

Proteins are essential for the construction of living organisms

regulation of physiological functions

and energy supply for life activities. With the introduction of proteomics technology and the rapid development of biotechnology

the demand for efficient protein separation is increasing. The development of effective protein separation techniques is essential to advance proteomics research and biotechnology applications. This review aims to summarize recent advances in the field of protein separation using polymeric materials

focusing on the design

function and application potential of these materials. The review begins with a classification of polymers according to their interaction mechanism with proteins

which includes size si

eving

hydrophobic interactions and electrostatic interactions. These interactions can be used for selective protein capture and purification. Neutral polymers are firstly introduced and classified into hydrophilic and hydrophobic polymers

which separate proteins

via

size sieving and hydrophobic interactions

and are known for their abilities in constructing stable three-dimensional network structures

reducing non-specific adsorption and maintaining protein activity. The second focus is placed on the separation of positively and negatively charged proteins by electrostatic attraction or repulsion

and the introduction of abundant charged functional groups on polymeric materials can provide active sites for protein binding. Tunable charge systems are also discussed

with a focus on composites that can be switched between cationic

neutral and anionic states. Weak polyelectrolytes that undergo reversible protonation and deprotonation are commonly used in the tunable system as their charge state changes in response to changes in pH or other environmental factors. This property facilitates the control of protein adsorption and desorption processes. The application of stimuli-responsive polymers with changeable physical or chemical properties in response to external stimuli such as temperature

light

or electric fields

in improving protein capture efficiency and selectivity is also reviewed. Finally

this review predicts future trends in protein separation technology

envisioning more efficient

precise

and intelligent systems capable of achieving higher purity and yield of protein separation. The development of cost-effective

scalable methods to meet the stringent requirements of personalized therapies and industrial biopharmaceutical production remains a challenge. The combination of information technology and traditional experimental science will pave the way for next-generation protein separation tools that address these challenges

making a significant contribution to the advancement of

life sciences and healthcare.

关键词

Keywords

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