拓扑结构聚丙烯酸对蛋白构象及功能的影响

叶安淇; 黎哲祺; 游世超; 陈浩林; 石毅; 刘利新; 陈永明

doi:10.11777/j.issn1000-3304.2026.26137

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拓扑结构聚丙烯酸对蛋白构象及功能的影响

研究论文 | 更新时间：2026-07-29

- 拓扑结构聚丙烯酸对蛋白构象及功能的影响
- Effects of Topological Poly(acrylic acid)s on Protein Conformation and Functions
- 高分子学报 2026年页码：1-18
- 作者机构：
  
  1.中山大学材料科学与工程学院聚合物复合材料及功能材料教育部重点实验室广州 510275
  2.河南大学化学与分子科学学院开封 475004
  3.河南大学抗病毒性传染病创新药物全国重点实验室郑州 450046
- 作者简介：
  
  E-mail: chenhlin58@mail.sysu.edu.cn;
- 基金信息：
  
  国家自然科学基金(基金号 ┣22075324);82341037┫
- DOI：10.11777/j.issn1000-3304.2026.26137
  中图分类号：
- CSTR：32057.14.GFZXB.2026.7646
- 收稿：2026-04-24，
  
  录用：2026-05-26，
  
  网络首发：2026-07-28，
- 稿件说明：
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叶安淇, 黎哲祺, 游世超, 陈浩林, 石毅, 刘利新, 陈永明. 拓扑结构聚丙烯酸对蛋白构象及功能的影响. 高分子学报, doi: 10.11777/j.issn1000-3304.2026.26137.

Ye, A. Q.; Li, Z. Q.; You, S. C.; Chen, H. L.; Shi, Y.; Liu, L. X.; Chen, Y. M. Effects of topological poly(acrylic acid)s on protein conformation and functions. Acta Polymerica Sinica (in Chinese), doi: 10.11777/j.issn1000-3304.2026.26137.
叶安淇, 黎哲祺, 游世超, 陈浩林, 石毅, 刘利新, 陈永明. 拓扑结构聚丙烯酸对蛋白构象及功能的影响. 高分子学报, doi: 10.11777/j.issn1000-3304.2026.26137. DOI： CSTR： 32057.14.GFZXB.2026.7646.

Ye, A. Q.; Li, Z. Q.; You, S. C.; Chen, H. L.; Shi, Y.; Liu, L. X.; Chen, Y. M. Effects of topological poly(acrylic acid)s on protein conformation and functions. Acta Polymerica Sinica (in Chinese), doi: 10.11777/j.issn1000-3304.2026.26137. DOI： CSTR： 32057.14.GFZXB.2026.7646.

摘要

蛋白质二级结构与其生物功能密切相关，研究聚合物与蛋白质相互作用及调控规律具有重要意义. 线型聚丙烯酸(PAA)可结合蛋白表面氨基并诱导构象转变，但不同拓扑结构PAA对蛋白构象和功能的调控尚缺乏系统研究. 本文采用“相同拓扑对比分子量、相近分子量对比拓扑结构”的策略，合成系列线型与分子刷型PAA

利用圆二色谱探究聚合物结构对蛋白的调控规律. 结果表明，对于低分子量蛋白，线型PAA诱导蛋白由

-螺旋向

-折叠转变，分子量越高构象平衡耗时越长. 相近分子量下，分子刷型PAA可加快该构象转变. 高分子量蛋白构象变化与PAA结构关联性较弱. 拓扑结构PAA对不同蛋白的功能影响不同：分子刷型PAA稳定

-半乳糖苷酶活性，而线型PAA破坏酶活. 小鼠免疫实验证实，线型PAA作为卵清蛋白(OVA)佐剂可诱导更高水平特异性IgG与干扰素-

. 机制上，分子刷引发抗原剧烈构象重塑易损伤表位，线型聚合物温和调控更利于保护功能性表位. 综上，聚合物分子量与拓扑结构是调控蛋白结构与功能的关键参数.

Abstract

The secondary structure of proteins is tightly linked to their biological functions

and investigating polymer-protein interactions and their regulatory rules is of great significance. Linear poly(acrylic acid) (PAA) binds to amino groups on protein surfaces and triggers conformational transitions. However

systematic studies on how PAA with distinct topologies modulates protein conformation and function remain scarce. This work adopts the strategy of comparing molecular weights under identical to

pologies and contrasting topologies at comparable molecular weights. A series of linear and bottlebrush PAA samples are synthesized

and circular dichroism spectroscopy is employed to explore how polymer structures regulate proteins. The results reveal that linear PAA induces the transition from

-helix to

-sheet in low-molecular-weight proteins; longer equilibrium times for conformational conversion are observed at higher PAA molecular weights. At similar molecular weights

bottlebrush PAA accelerates this conformational transition. In contrast

the conformational changes of high-molecular-weight proteins show weak correlation with PAA structures. PAA topologies exert divergent effects on different proteins: bottlebrush PAA stabilizes

-galactosidase activity

whereas linear PAA impairs it. Mouse immunization assays verify that linear PAA acts as an adjuvant for ovalbumin (OVA) to elicit elevated levels of antigen-specific IgG and interferon-

. Mechanistically

drastic antigen conformational remodeling induced by bottlebrush polymers tends to damage critical epitopes

while mild

gradual regulation by linear polymers better preserves functional epitopes. In summary

the molecular weight and topology of polymers serve as key parameters governing protein structure and function.

关键词

Keywords

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