A novel kind of biodegradable glucose-sensitive polyphosphazene hydrogel has been prepared basing on both the chemical crosslinking and the specific interaction between polymerbound glucose and concanavalin A (Con A).Glucosyl-co-methoxyethoxyl-co-2-(2-aminoethoxy)ethoxyl substituted polyorgano-phosphazene was synthesized by subsequently nucleophilic substitution of polydichlorophosphazene with 2-［2-((tert-butoxycarbony1)amino)ethoxy］ethanol，diisopropylidene D-glucose and methoxyethanol，which was followed by deprotection treatment with trifluoroacetic acid to restore glycosyl and free amino groups.A chemical crosslinked hydrogel was obtained by reacting the above polymer with glutaraldehyde.The polyphazene hydrogel showed good swelling behavior in phosphate buffer solutions，and exhibited glucose-sensitive water absorption after Con A being loaded.No macroscopic deformation could be observed by immersing the Con A-loaded polyphoaphzene hydrogel into aqueous solution with high content of glucose for the presence of chemical crosslinking.The release rate of insulin from this polyphosphazene hydrogel depended on the concentration of free glucose in medium obviously.As the glucose concentration was increased from 1 mg/mL to 4 mg/mL，the release rate of insulin from the hydrogel increased，whereas，the release rate decreased when the glucose concentration was reduced to 1 mg/mL.The results demonstrated the possibility of using chemical crosslinked glucose-bearing polyphosphazene to prepare glucose-sensitive hydrogels in combination with Con A and to regulate the insulin release as a function of the free glucose concentration in the environment.